Programmed cell death 1; also known as PDCD1; is a type I transmembrane glycoprotein; and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase; SHP-2 upon interacting with either of two ligands; PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1; IL-4; IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition; coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance; and thus contributes to the prevention of autoimmune diseases. Furthermore; PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule; PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation; cytokine production; and cytolytic function.Immune CheckpointImmune Checkpoint Blockade: Blocking Antibodies Immune Checkpoint Blockade: PD1 / PDCD1 / CD279 Blocking AntibodiesImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IHC Antibodies Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint Proteins PD1 / PDCD1 / CD279 Immune Checkpoint ProteinsPD1 / PDCD1 / CD279 Immune Checkpoint PD1 / PDCD1 / CD279 Immune Checkpoint AntibodiesImmune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Human PD/PDCD1 Recombinant Protein (RPES3752)
Programmed cell death protein 1,PDCD1,PD-1,hPD-1,CD279,SLEB2,Hsle1
Met 1-Gln 167
C-His & Fc
Measured by its binding ability in a functional ELISA. Immobilized recombinant human PD-L2 at 1 µg/ml (100ul/well) can bind human PD1 / Fc chimera with a linear range of 7.8-1000 ng/ml.
<1.0 EU per µg as determined by the LAL method.
A DNA sequence encoding the extracellular domain (Met 1-Gln 167) of human PD1 (NP_005009.2) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
> 97 % as determined by reducing SDS-PAGE.
AP Mol Mass:
Lyophilized from sterile PBS; pH 7.4
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.