Ras-related C3 botulinum toxin substrate 2 (Rac2) is a small G-protein belonging to the Ras subfamily of the GTPase family. Rac2 acts as an "on / off" switch for signal transduction cascades and motilities. When GDP is attached to the small G-protein, the enzyme is inactivated. Release of the GDP and replace of the GTP cativate the GTPasee. Rac2 remains active until the GTP is hydrolyzed to GDP. Rac2 is a hematopoietic-specific Rho family GTPase implicated as an important constituent of the NADPH oxidase complex and shares 92% amino acid identity with the ubiquitously expressed Rac1. The small G-protein Rac2 regulates the rearrangements of actin and membrane necessary for Fcy receptor-mediated phagocytosis by macrophages. Activated Rac2 binds to the p21-binding domain of PAK1 and this binding provided a basis for microscopic methods to localize activation of these G proteins inside cells.
Product Name:
Human RAC2 Recombinant Protein (RPES3620)
Product Code:
RPES3620
Size:
20µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
EN-7,Gx,HSPC022,p21-Rac2
Accession:
P15153
Sequence:
Met 1-Cys 189
Fusion tag:
N-His
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human RAC2 (P15153) (Met 1-Cys 189) was expressed, with a polyhistidine tag at the N-terminus.
Purity:
> 94 % as determined by reducing SDS-PAGE.
Mol Mass:
22.6 kDa
AP Mol Mass:
25 kDa
Formulation:
Lyophilized from sterile PBS, 20% glycerol, pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
