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Recombinant Human SARS-CoV-2 Spike RBD Variant 501Y.V2, Lineage B.1.351, SA (HEK)

SKU:
DC0007151
  • Recombinant Human SARS-CoV-2 Spike RBD Variant 501YV2, Lineage B.1.351, SA HEK
  • Recombinant Human SARS-CoV-2 Spike RBD Variant 501YV2, Lineage B.1.351, SA HEK
€2,200
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Description

Recombinant Human SARS-CoV-2 Spike RBD Variant 501Y.V2, Lineage B.1.351 (HEK)

Background:

Spike protein (S protein) is one of four structural proteins of Coronavirus (SARS-Cov, SARS-Cov-2, MERS amongst other), S protein plays the most important role in viral attachment, fusion and entry, and it serves as a target for development of antibodies, entry inhibitors and vaccines. In the S protein, the Receptor Binding Domain (RBD) mediates viral entry of SARS-Cov and SARS-Cov-2 into host cells by its interaction with the membrane receptor ACE2 (Angiotensin-converting enzyme 2). Recently, the variant 20C/501Y.V2 also named 501Y.V2 (Lineage B.1.351) has been first identified in South Africa in the middle of December 2020. It has been reported the 501Y.V2 variant causes more frequently serious illness in such cases than other variants.

Description

 
Product Name: Recombinant Human SARS-CoV-2 Spike RBD Variant 501Y.V2, Lineage B.1.351 (HEK)
Synonym: 20C/501Y.V2 (South Africa)
Sequence: Native NCBI Accession Number: MN908947 Arg319-Phe541, with mutation K417N; E484K; N501Y
Expression System: HEK293 cells
Tag: HIS Tag C-Terminus
Purification: Affinity chromatography
Extinction coefficient: 35340 M-1.cm-1 Abs 0.1% (=1 g/l) 1.35 assuming all pairs of Cys residues form cystines
Predicted Molecular Weight: 26.1 kDa

Specifications

 
SDS Page: Approx. 32 kDa
Size: 100µg
Purity: >95% by SDS PAGE
Formulation: Lyophilized from PBS (Na2HPO4/NaH2PO4 50mM pH 7,2, NaCl 150mM, Trehalose)
Reconstitution: Centrifuge the vial prior opening. It is recommended to reconstitute in sterile water at 100 µg/ml
Activity: Recognized by CR3022 anti RBD recombinant antibody and Covid-19 positive patients serum samples.
Stability and Storage: Store at minimum -20°C. Avoid repeated freeze-thaw cycles Stable 1 month -20°C to -80°C after reconstitution under sterile conditions

Recombinant Human SARS-CoV-2 Spike RBD Variant 501Y.V2, Lineage B.1.351 (HEK) Images

SA1 SDS-PAGE 15% under reducing conditions and visualized by Coomassie blue staining showing a band at approx. 32 kDa
SA2 Western Blot: RBD Variant 501Y.V2 HIS Tag detected with anti-HIS-Tag antibody HRP conjugate showing a band at approx. 32 kDa