Recombinant Human SARS-CoV-2 Spike RBD Variant 501Y.V3, Lineage B.1.1.248, Brazil & Japan (HEK)

SKU:
DC0007152
  • Recombinant Human SARS-CoV-2 Spike RBD Variant 501YV3, Lineage B.1.1.248, Brazil and Japan HEK
  • Recombinant Human SARS-CoV-2 Spike RBD Variant 501YV3, Lineage B.1.1.248, Brazil and Japan HEK
€1,760

Description

Recombinant Human SARS-CoV-2 Spike RBD Variant 501Y.V3, Lineage B.1.1.248, Brazil & Japan (HEK)

Background:

Spike protein (S protein) is one of four structural proteins of Coronavirus (SARS-Cov, SARS-Cov-2, MERS amongst other), S protein plays the most important role in viral attachment, fusion and entry, and it serves as a target for development of antibodies, entry inhibitors and vaccines. In the S protein, the Receptor Binding Domain (RBD) mediates viral entry of SARS-Cov and SARS-Cov-2 into host cells by its interaction with the membrane receptor ACE2 (Angiotensin-converting enzyme 2). This variant 501Y.V3 also named P1 (20J/501Y.V3) of SARS-CoV-2 was first identified in Japan on early January 2021 detected in four travelers from Brazil, and rapidly declared to be circulating in Brazil.

Description

 
Product Name: Recombinant Human SARS-CoV-2 Spike RBD Variant 501Y.V3 Lineage B.1.1.248 (HEK)
Synonym: P1 20J/501Y.V3 (Brazil & Japan)
Sequence: Native NCBI Accession Number MN908947, Arg319-Phe541, with mutation K417T; E484K; N501Y
Expression System: HEK293 cells
Tag: HIS Tag C-Terminus
Purification: Affinity chromatography
Extinction coefficient: 35340 M-1.cm-1 Abs 0.1% (=1 g/l) 1.35 assuming all pairs of Cys residues form cystines
Predicted Molecular Weight: 26.1 kDa

Specifications

 
SDS Page: Approx. 32 kDa
Concentration: 1mg/ml
Purity: >95% by SDS PAGE
Formulation: Liquid PBS
Activity: Recognized by CR3022 anti RBD recombinant antibody and Covid-19 positive patients serum samples.
Stability and Storage: Store at minimum -20°C. Avoid repeated freeze-thaw cycles

Recombinant Human SARS-CoV-2 Spike RBD Variant 501Y.V3, Lineage B.1.1.248, Brazil & Japan (HEK) Images

BJ1 SDS-PAGE 4-15% under reducing conditions and visualized by Coomassie blue staining showing a band at approx. 32 kDa
BJ2 Western Blot: RBD Variant 501Y.V3 HIS Tag detected with anti-HIS-Tag antibody HRP conjugate showing a band at approx. 32 kDa
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