Reduced Glutathione (GSH) Assay Kit (Fluorometric) (BN00956)

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BN00956
  • Reduced Glutathione (GSH) Assay Kit (Fluorometric) (BN00956)
  • Reduced Glutathione (GSH) Assay Kit (Fluorometric) (BN00956)
  • Reduced Glutathione (GSH) Assay Kit (Fluorometric) (BN00956)
  • Reduced Glutathione (GSH) Assay Kit (Fluorometric) (BN00956)
€673

Description

ELISA Kit Technical ManualMSDS

Reduced Glutathione (GSH) Assay Kit

Glutathione (GSH), a thiol-containing tripeptide, is a key antioxidant in living systems. Intracellular GSH status appears to be a sensitive indicator of the overall health of a cell. Assay Genie's Reduced Glutathione Assay Kit is based on a specific enzymatic cycling method in the presence of GSH and a fluorophore. The reduction of the fluorophore produces a stable fluorescent product, the fluorescence of which is directly proportional to the amount of GSH in the sample and can be followed kinetically (Ex/Em=535/587 nm). Our assay is the most specific and sensitive in the market. Oxidized Glutathione does not interfere with the assay. The assay is simple, reproducible and can specifically detect as low as 8 pmol/well of reduced form of Glutathione (GSH) in a 100 µl reaction.

Figure: (a) GSH Standard Curve, results from multiple experiments. (b) Assay Specificity: measurement of GSH (100 pmol) and GSSG (1000 pmol). The kit can effectively discriminate reduced GSH and oxidized GSSG forms. (c) Measurement of GSH in Human Plasma (Spiked and Unspiked samples) (d) Measurement of GSH in Mouse Liver (1.2 µg protein), HepG2 Cell Extract (0.6 µg protein), Jurkat Cell Extract (1 µg protein) and Human Plasma (10 µl, 1:4 dilution). All assays were performed following kit protocol.

Key Information Description

Product SKU

BN00956

Size

100 assays

Kit Summary

  • Detection method- Fluorescence (535/587 nm)
  • Species reactivity- Mammalian
  • Applications- The Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.

Detection Method

Detection method- Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

The Fluorometric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.

Features and Benefits

  • Simple procedure; takes only ~1-2 hours
  • Fast and convenient
  • Specific: does not detect oxidized GSSG
  • Sensitive: detects as low as 8 pmol GSH

Kit Components

  • GSH Assay Buffer (in DMSO)
  • Substrate Mix A
  • Substrate Mix B
  • Enzyme Mix A
  • Enzyme Mix B
  • Enzyme Mix C
  • Sulfosalicylic Acid (SSA, 1 gram)
  • GSH Standard

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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