Renin Inhibitor Screening Kit (Fluorometric) (BN01015)

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SKU:
BN01015
€770

Description

ELISA Kit Technical ManualMSDS

Renin Inhibitor Screening Kit (Fluorometric)

Renin (EC 3.4.23.15), also known as an angiotensinogenase, is an enzyme that participates in the renin-angiotensin system (RAS) which mediates extracellular volume (i.e. blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. An over-active renin-angiotensin system leads to vasoconstriction and retention of sodium and water, causing hypertension. Renin inhibitors are widely used for the treatment of hypertension. Assay Genie's Renin inhibitor screening Kit uses a synthetic peptide substrate with a fluorophore (EDANS) at one end and a quencher (DABCYL) at the other end. Renin catalyzes the cleavage of FRET substrate resulting in a product that is detected fluorometrically at Ex/Em = 328/552 nm. In the presence of a Renin inhibitor, the rate of hydrolysis of the substrate is decreased. The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Renin inhibitors also adaptable to a 384 well format.

Figure: Inhibition of Renin Enzyme Activity with Inhibitor Aliskiren using kit protocol.

Key Information Description

Product SKU

BN01015

Size

100 assays

Detection Method

v

Applications

  • Screening potential inhibitors of renin
  • Characterize/study renin inhibitors

Features and Benefits

  • Rapid & simple
  • Reliable test for screening potential inhibitors of renin
  • Includes Inhibitor Control [Aliskiren]

Kit Components

  • Renin Assay Buffer
  • Renin Substrate
  • Active Human Renin (lyophilized) (15 µg)
  • Inhibitor Control [Aliskiren] (200 µM)

Storage Conditions

-80°C

Shipping Conditions

Dry Ice

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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