Anti-RNF135 Antibody (CAB9661)

Antibody Name:	Anti-RNF135 Antibody
Antibody SKU:	CAB9661
Antibody Size:	20µL, 50µL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 51-300 of human RNF135 (NP_115698.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	293T, THP-1, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 51-300 of human RNF135 (NP_115698.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ARDA RRWA CPTC RQGA AQQP HLRK NTLL QDLA DKYR RAAR EIQA GSDP AHCP CPGS SSLS SAAA RPRR RPEL QRVA VEKS ITEV AQEL TELV EHLV DIVR SLQN QRPL SESG PDNE LSIL GKAF SSGV DLSM ASPK LVTS DTAA GKIR DILH DLEE IQEK LQES VTWK EAPE AQMQ GELL EAPS SSSC PLPD QSHP ALRR ASRF AQWA IHPT FNLK SLSC SLEV SKDS RTVT VSHR PQPY RWSC ERFS TS
Gene ID:	84282
Uniprot:	Q8IUD6
Cellular Location:	Cytoplasm
Calculated MW:	22kDa/30kDa/47kDa
Observed MW:	48kDa

Synonyms:

RNF135, L13, MMFD, REUL, Riplet

Background:

The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene is located in a chromosomal region known to be frequently deleted in patients with neurofibromatosis. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

UniProt Protein Function:	RNF135: Acts as an E2-dependent E3 ubiquitin-protein ligase, involved in innate immune defense against viruses. Ubiquitinates DDX58 and is required for full activation of the DDX58 signaling resulting in interferon beta production. Defects in RNF135 are the cause of macrocephaly macrosomia facial dysmorphism syndrome (MMFD). MMFD is an autosomal dominant disorder characterized by the association of macrothrombocytopathy and progressive sensorineural hearing loss without renal dysfunction. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA replication; Calcium-binding; Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.- Chromosomal Location of Human Ortholog: 17q11.2 Cellular Component: cytoplasm; cytosol Molecular Function:protein binding; ribonucleoprotein binding; ubiquitin-protein ligase activity Biological Process: innate immune response; negative regulation of interferon type I production; positive regulation of interferon-beta production; protein ubiquitination; regulation of innate immune response Disease: Macrocephaly, Macrosomia, Facial Dysmorphism Syndrome
NCBI Summary:	The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene is located in a chromosomal region known to be frequently deleted in patients with neurofibromatosis. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8IUD6
NCBI GenInfo Identifier:	269849639
NCBI Gene ID:	84282
NCBI Accession:	Q8IUD6.2
UniProt Secondary Accession:	Q8IUD6,Q9BSE9, A0AVM5, B2R7G9, B6ZLM5, F5GX60,
UniProt Related Accession:	Q8IUD6
Molecular Weight:	30,989 Da
NCBI Full Name:	E3 ubiquitin-protein ligase RNF135
NCBI Synonym Full Names:	ring finger protein 135
NCBI Official Symbol:	RNF135
NCBI Official Synonym Symbols:	L13; MMFD; REUL; Riplet
NCBI Protein Information:	E3 ubiquitin-protein ligase RNF135
UniProt Protein Name:	E3 ubiquitin-protein ligase RNF135
UniProt Synonym Protein Names:	RIG-I E3 ubiquitin ligase; REUL; RING finger protein 135; Riplet
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	RNF135
UniProt Entry Name:	RN135_HUMAN

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)