Anti-ROBO1 Antibody (CAB15313)

Antibody Name:	Anti-ROBO1 Antibody
Antibody SKU:	CAB15313
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1452-1651 of human ROBO1 (NP_002932.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, U-251MG, HeLa, 293T, U-251MG, HeLa, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1452-1651 of human ROBO1 (NP_002932.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AAVM QKTR PAKK LKHQ PGHL RRET YTDD LPPP PVPP PAIK SPTA QSKT QLEV RPVV VPKL PSMD ARTD RSSD RKGS SYKG REVL DGRQ VVDM RTNP GDPR EAQE QQND GKGR GNKA AKRD LPPA KTHL IQED ILPY CRPT FPTS NNPR DPSS SSSM SSRG SGSR QREQ ANVG RRNI AEMQ VLGG YERG EDNN EELE ETES
Gene ID:	6091
Uniprot:	Q9Y6N7
Cellular Location:	Cell membrane, Cell projection, Single-pass type I membrane protein, axon
Calculated MW:	169kDa/175kDa/176kDa/180kDa/181kDa
Observed MW:	270kDa

Synonyms:

ROBO1, DUTT1, SAX3

Background:

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ROBO1: Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development. Belongs to the immunoglobulin superfamily. ROBO family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral Chromosomal Location of Human Ortholog: 3p12 Cellular Component: cell surface; cytoplasm; integral to plasma membrane; plasma membrane Molecular Function:axon guidance receptor activity; identical protein binding; LRR domain binding; protein binding Biological Process: axon guidance; axon midline choice point recognition; caspase activation; cell adhesion; cell migration during sprouting angiogenesis; chemorepulsion involved in postnatal olfactory bulb interneuron migration; homophilic cell adhesion; negative regulation of cell migration; negative regulation of mammary gland epithelial cell proliferation; negative regulation of negative chemotaxis; nervous system development; positive regulation of axonogenesis; positive regulation of Rho protein signal transduction
NCBI Summary:	Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Code:	Q9Y6N7
NCBI GenInfo Identifier:	49036500
NCBI Gene ID:	6091
NCBI Accession:	Q9Y6N7.1
UniProt Secondary Accession:	Q9Y6N7,Q1RMC7, Q7Z300, Q9BUS7, B2RXI1, D3DU36, E9PD49
UniProt Related Accession:	Q9Y6N7
Molecular Weight:	169,661 Da
NCBI Full Name:	Roundabout homolog 1
NCBI Synonym Full Names:	roundabout guidance receptor 1
NCBI Official Symbol:	ROBO1
NCBI Official Synonym Symbols:	SAX3; DUTT1
NCBI Protein Information:	roundabout homolog 1
UniProt Protein Name:	Roundabout homolog 1
UniProt Synonym Protein Names:	Deleted in U twenty twenty; H-Robo-1
Protein Family:	Roundabout
UniProt Gene Name:	ROBO1
UniProt Entry Name:	ROBO1_HUMAN

	Western blot analysis of extracts of various cell lines, using ROBO1 antibody (CAB15313) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Western blot analysis of extracts of various cell lines, using ROBO1 antibody (CAB15313) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of U-2 OS cells using ROBO1 Polyclonal Antibody (CAB15313) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-ROBO1 Antibody (CAB9412)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)