Anti-RPS7 Antibody (CAB6731)

Antibody Name:	Anti-RPS7 Antibody
Antibody SKU:	CAB6731
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human RPS7 (NP_001002.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, NIH/3T3, SW480, MCF7, A-549, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human RPS7 (NP_001002.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MFSS SAKI VKPN GEKP DEFE SGIS QALL ELEM NSDL KAQL RELN ITAA KEIE VGGG RKAI IIFV PVPQ LKSF QKIQ VRLV RELE KKFS GKHV VFIA QRRI LPKP TRKS RTKN KQKR PRSR TLTA VHDA ILED LVFP SEIV GKRI RVKL DGSR LIKV HLDK AQQN NVEH KVET FSGV YKKL TGKD VNFE FPEF QL
Gene ID:	6201
Uniprot:	P62081
Cellular Location:	Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	22kDa
Observed MW:	24kDa

Synonyms:

RPS7, DBA8, S7

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S7E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPS7: Required for rRNA maturation. Defects in RPS7 are the cause of Diamond-Blackfan anemia type 8 (DBA8). DBA8 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein S7e family.
UniProt Protein Details:	Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 2p25 Cellular Component: small subunit processome; focal adhesion; membrane; cytoplasm; nucleolus; ribosome; microtubule organizing center; cytosol; ribonucleoprotein complex; nucleus Molecular Function:protein binding; structural constituent of ribosome; RNA binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; translation; viral reproduction; translational termination; viral infectious cycle; ribosomal small subunit biogenesis and assembly; translational elongation; cellular protein metabolic process; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; rRNA processing Disease: Diamond-blackfan Anemia 8
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S7E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P62081
NCBI GenInfo Identifier:	49065831
NCBI Gene ID:	6201
NCBI Accession:	P62081.1
UniProt Secondary Accession:	P62081,P23821, P24818, Q57Z92, Q6IPH1,
UniProt Related Accession:	P62081
Molecular Weight:	194
NCBI Full Name:	40S ribosomal protein S7
NCBI Synonym Full Names:	ribosomal protein S7
NCBI Official Symbol:	RPS7
NCBI Official Synonym Symbols:	S7; DBA8
NCBI Protein Information:	40S ribosomal protein S7
UniProt Protein Name:	40S ribosomal protein S7
Protein Family:	Ribosomal protein
UniProt Gene Name:	RPS7
UniProt Entry Name:	RS7_HUMAN

	Western blot analysis of extracts of various cell lines, using RPS7 antibody (CAB6731) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using RPS7 antibody (CAB6731) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast using RPS7 antibody (CAB6731) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spinal cord using RPS7 antibody (CAB6731) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using RPS7 antibody (CAB6731). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)