SARS-Cov-2 Nucleocapsid Monoclonal Antibody [R001]

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SKU:
AGEL2117
€623

Description

SARS-CoV-2 N Protein - Background Information

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

SARS-CoV-2 N Antibody Properties

Information Description

Immunogen

Recombinant 2019-nCoV Nucleoprotein / NP Protein

Source

Rabbit

Reactivity

SARS-CoV-2

Application

WB, ELISA

Concentration

1 mg/mL

Recommended Dilution

WB: 1:1,000-1:5,000
ELISA: 1:5,000-1:10,000

Clone No.

R001

Isotype

IgG

Conjugation

Unconjugated

Storage

Store at -20℃. Avoid freeze / thaw cycles. Recommended storage buffer: 0.2 μm filtered solution of PBS.

SARS-CoV-2 Nucleocapsid Information

Key Information Description

Uniprot:

Protein:

Nucleoprotein

Gene:

N

Organism:

Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)

Alternative Names:

Nucleocapsid protein, Nucleoprotein, NC, Protein N

Nucleocapsid function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed:17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed:18055455).

Sub-cellular location

    Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding.

Post-Translational Modification

ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.

Phosphorylated on serine and threonine residues (By similarity). Phosphorylated by host GSK3A and GSK3B. Phosphorylation allows recruitment of host RNA helicase DDX1 which facilitates template readthrough and enables longer subgenomic mRNA synthesis.

Interaction

Homooligomer. Both monomeric and oligomeric forms interact with RNA.

Interacts with protein M (PubMed:15351485).

Interacts with protein E (PubMed:24766657). May bind to host HNRNPA1 (Probable).

Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection (By similarity). May interact with host SMAD3 (Probable).

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