Anti-SDHB Antibody (CAB10821)

Antibody Name:	Anti-SDHB Antibody
Antibody SKU:	CAB10821
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-280 of human SDHB (NP_002991.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, HeLa, U-87MG, Mouse brain, Mouse liver, Mouse kidney, Mouse heart, Rat liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-280 of human SDHB (NP_002991.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AQTA AATA PRIK KFAI YRWD PDKA GDKP HMQT YEVD LNKC GPMV LDAL IKIK NEVD STLT FRRS CREG ICGS CAMN INGG NTLA CTRR IDTN LNKV SKIY PLPH MYVI KDLV PDLS NFYA QYKS IEPY LKKK DESQ EGKQ QYLQ SIEE REKL DGLY ECIL CACC STSC PSYW WNGD KYLG PAVL MQAY RWMI DSRD DFTE ERLA KLQD PFSL YRCH TIMN CTRT CPKG LNPG KAIA EIKK MMAT YKEK KASV
Gene ID:	6390
Uniprot:	P21912
Cellular Location:	Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:	31kDa
Observed MW:	32kDa

Synonyms:

SDHB, CWS2, IP, PGL4, SDH, SDH1, SDH2, SDHIP

Background:

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.

UniProt Protein Function:	SDHB: Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC). A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent. Defects in SDHB are the cause of paragangliomas type 4 (PGL4). A neural crest tumor usually derived from the chromoreceptor tissue of a paraganglion. Paragangliomas are most commonly located in the head and neck region, specifically at the carotid bifurcation, the jugular foramen, the vagal nerve, and in the middle ear. Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma (PGGSS); also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance. Defects in SDHB are a cause of Cowden-like syndrome (CWDLS). Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus. Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.
UniProt Protein Details:	Protein type:EC 1.3.5.1; Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; Oxidoreductase; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 1p36.1-p35 Cellular Component: mitochondrial respiratory chain complex II; mitochondrion; mitochondrial inner membrane Molecular Function:2 iron, 2 sulfur cluster binding; ubiquinone binding; succinate dehydrogenase (ubiquinone) activity; protein binding; electron carrier activity; metal ion binding; 4 iron, 4 sulfur cluster binding; 3 iron, 4 sulfur cluster binding Biological Process: succinate metabolic process; cellular metabolic process; tricarboxylic acid cycle; aerobic respiration Disease: Gastrointestinal Stromal Tumor; Cowden Syndrome 2; Paraganglioma And Gastric Stromal Sarcoma; Paragangliomas 4; Pheochromocytoma
NCBI Summary:	Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq, Jul 2008]
UniProt Code:	P21912
NCBI GenInfo Identifier:	20455488
NCBI Gene ID:	6390
NCBI Accession:	P21912.3
UniProt Related Accession:	P21912
Molecular Weight:
NCBI Full Name:	Succinate dehydrogenase
NCBI Synonym Full Names:	succinate dehydrogenase complex iron sulfur subunit B
NCBI Official Symbol:	SDHB
NCBI Official Synonym Symbols:	IP; SDH; CWS2; PGL4; SDH1; SDH2; SDHIP
NCBI Protein Information:	succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
UniProt Protein Name:	Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
UniProt Synonym Protein Names:	Iron-sulfur subunit of complex II; Ip
Protein Family:	Succinate dehydrogenase
UniProt Gene Name:	SDHB
UniProt Entry Name:	SDHB_HUMAN

	Western blot analysis of extracts of various cell lines, using SDHB antibody (CAB10821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human colon using SDHB antibody (CAB10821) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using SDHB antibody (CAB10821) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat spleen using SDHB antibody (CAB10821) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using SDHB Rabbit pAb (CAB10821) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-SDHB Antibody (CAB1809)

SDHB Antibody (PACO02126)

SDHB Antibody (PACO12074)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)