Senescence Detection Kit (Fluorometric) (BN01149)

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SKU:
BN01149
€816

Description

ELISA Kit Technical ManualMSDS

Senescence Detection Kit (Fluorometric)

Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. Senescence represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or passage in culture. Senescent cells display increase of cell size, senescence-associated expression of Beta-galactosidase (SA-Beta-Gal) activity, and altered patterns of gene expression. The Senescence detection kit is designed to fluorescently detect SA-Beta-Gal activity in cultured cells by FACS. The SA-Beta-Gal is present only in senescent cells and is not found in pre-senescent, quiescent or immortal cells.

Figure: 3T3 cells were plated at 5x105 cells per well in a 24well, treated for 4 hours (with and without 200nM of Daunorubicin. HCl; test and control response respectively) in complete cell culture media for 48 hours at 37°C/5% CO2. Media was removed and replaced with media containing Senescence Dye and incubated for 2 hours at 37°C/5% CO2. After incubation time, cells were washed 2x with Wash Buffer, the cells were trypsinized, washed once with Wash Buffer and analyzed by FACS.

Key Information Description

Product SKU

BN01149

Assay Size

100 assays

Detection

FACS (FL1 channel)

Reactivity

Mammalian

Application

Detection of senescence cells

Features and Benefits

Simple, one step protocol

Kit Contents

  • Wash Buffer
  • Senescence Dye

Storage

-20°C

Shipping Conditions

Gel Pack

Research Use

For Research Use Only! Not For Use in Humans.

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