Senescence Detection Kit (Fluorometric) (BN01149)
- SKU:
- BN01149
Description
Senescence Detection Kit (Fluorometric)
Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. Senescence represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or passage in culture. Senescent cells display increase of cell size, senescence-associated expression of Beta-galactosidase (SA-Beta-Gal) activity, and altered patterns of gene expression. The Senescence detection kit is designed to fluorescently detect SA-Beta-Gal activity in cultured cells by FACS. The SA-Beta-Gal is present only in senescent cells and is not found in pre-senescent, quiescent or immortal cells.
Figure: 3T3 cells were plated at 5x105 cells per well in a 24well, treated for 4 hours (with and without 200nM of Daunorubicin. HCl; test and control response respectively) in complete cell culture media for 48 hours at 37°C/5% CO2. Media was removed and replaced with media containing Senescence Dye and incubated for 2 hours at 37°C/5% CO2. After incubation time, cells were washed 2x with Wash Buffer, the cells were trypsinized, washed once with Wash Buffer and analyzed by FACS.
| Key Information | Description |
| Product SKU | BN01149 |
| Assay Size | 100 assays |
| Detection | FACS (FL1 channel) |
| Reactivity | Mammalian |
| Application | Detection of senescence cells |
| Features and Benefits | Simple, one step protocol |
| Kit Contents |
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| Storage | -20°C |
| Shipping Conditions | Gel Pack |
| Research Use | For Research Use Only! Not For Use in Humans. |
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