Smad2 Polyclonal Antibody
- SKU:
- CAB0440
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- IHC
Description
| Product Name: | Smad2 Polyclonal Antibody |
| Product Code: | CAB0440 |
| Reactivity: | Human |
| Applications: | Western blotting, Immunohistochemistry |
| Host Species: | Rabbit |
| Purification Method: | Affinity purification |
| Isotype: | IgG |
| Reactivity: | Human |
| Tested Applications: | WB, IHC-P, ELISA |
| Key Applications: | Western blotting, Immunohistochemistry |
| Recommended Dilution: | WB 1:500-1:1000, IHC-P 1:50-1:200 |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Positive Samples: | HeLa |
| Cellular Location: | Cytoplasm, Nucleus |
| Customer Validation: | WB(U87MG, Toxoplasma gondii, Rattus norvegicus, Homo sapiens) |
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 175-270 of human Smad2 (NP_005892.1). |
| Sequence: | LPPVL VPRHT EILTE LPPLD DYTHS IPENT NFPAG IEPQS NYIPE TPPPG YISED GETSD QQLNQ SMDTG SPAEL SPTTL SPVNH SLDLQ PVTYS E |
| Synonyms: | JV18, LDS6, CHTD8, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2 |
| Calculated MW: | 52kDa |
| Observed MW: | 50kDa |
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