Anti-SOCS7 Antibody (PACO51974)

Antibody Name:	SOCS7 Antibody (PACO51974)
Antibody SKU:	PACO51974
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IF:1:50-1:200
Species Reactivity:	Human, Rat
Immunogen:	Recombinant Human Suppressor of cytokine signaling 7 protein (6-165AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, U87 whole cell lysate, Rat heart tissue, Rat liver tissue. All lanes: SOCS7 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 63, 38 kDa. Observed band size: 63 kDa..
	Immunofluorescent analysis of HepG2 cells using PACO51974 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Regulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).

Synonyms:

Suppressor of cytokine signaling 7 (SOCS-7) (Nck, Ash and phospholipase C gamma-binding protein) (Nck-associated protein 4) (NAP-4), SOCS7, NAP4 SOCS6

UniProt Protein Function:	SOCS7: Regulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF- like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Motility/polarity/chemotaxis; InhibitorChromosomal Location of Human Ortholog: 17q12Cellular Component: cytoplasmMolecular Function: protein binding; protein kinase inhibitor activityBiological Process: cytokine and chemokine mediated signaling pathway; negative regulation of JAK-STAT cascade; negative regulation of protein kinase activity
UniProt Protein Details:
NCBI Summary:
UniProt Code:	O14512
NCBI GenInfo Identifier:	118572723
NCBI Gene ID:	30837
NCBI Accession:	O14512.2
UniProt Secondary Accession:	O14512,Q0IJ63, A2VCU2
UniProt Related Accession:	O14512
Molecular Weight:	37,461 Da
NCBI Full Name:	Suppressor of cytokine signaling 7
NCBI Synonym Full Names:	suppressor of cytokine signaling 7
NCBI Official Symbol:	SOCS7
NCBI Official Synonym Symbols:	NAP4; NCKAP4
NCBI Protein Information:	suppressor of cytokine signaling 7
UniProt Protein Name:	Suppressor of cytokine signaling 7
UniProt Synonym Protein Names:	Nck, Ash and phospholipase C gamma-binding protein; Nck-associated protein 4; NAP-4
Protein Family:	Suppressor of cytokine signaling
UniProt Gene Name:	SOCS7
UniProt Entry Name:	SOCS7_HUMAN

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)