Sodium Assay Kit (Colorimetric) (BN00649)

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BN00649
  • Sodium Assay Kit (Colorimetric) (BN00649)
  • Sodium Assay Kit (Colorimetric) (BN00649)
  • Sodium Assay Kit (Colorimetric) (BN00649)
€876

Description

ELISA Kit Technical ManualMSDS

Sodium Assay Kit (Colorimetric)

Sodium (Na) is one of the most important electrolytes along with chloride, calcium and potassium. Na plays vital roles in the maintenance of plasma volume, pH balance, transmission of nerve impulses, and normal cell functions. Healthy individuals can absorb sodium ingested in food, and kidneys maintain proper sodium balance by excreting its excess in urine. Sodium sources include table salt, milk, meat, shellfish, bread, snack food, etc. Normal Sodium intake has been defined to be between 200-500 mg/day. Patients suffering high blood pressure, hypertension, chronic kidney disease, and people suffering salt sensitivity require restricted low-sodium diets due to those conditions. Hyponatremia (low sodium concentration in blood) can occur in patients with nephrotic syndrome, excessive vomiting and diarrhea, while Hypernatremia (high sodium concentration in blood) is developed in patients suffering from liver diseases, burns, and pregnancy. Traditionally, sodium concentration in clinical settings is determined by potentiometric, gravimetry, photometry, titrimetry and flame atomic emission spectroscopy, but these methods require expensive and complex protocols that need to be performed by trained personnel. Assay Genie's Sodium Assay Kit offers a simple, two-step colorimetric assay that is based on the requirement of sodium ion as a cofactor for the enzymatic activity of Beta-Galactosidase (BetaG). Endogenous mono-, di-, and trivalent ions, ascorbic acid, creatinine, glucose, urea, and bilirubin do not interfere with the assay. The kit can detect Sodium concentration as low as 25 µM in a variety of samples.

Figure: (a) Sodium Standard Curve. (b) Assay Specificity: Sodium, and other mono, di and trivalent cations (15 nmol/0.15 mM each) were tested to evaluate possible interferences. Interferences were found to be less than 10% when data was normalized using Sodium as 100% activity. (c) Estimation of sodium in human pooled serum Off-the-Clot (5 µl; 50 times diluted), and human Urine (10 µl; 100 times diluted). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00649

Size

100 assays

Detection Method

Absorbance (405 nm)

Applications

The kit can detect Sodium concentration as low as 25 µM in a variety of samples.

Features and Benefits

  • Simple procedure; takes ~ 3 hours
  • Fast and convenient
  • The assay is sensitive and stable
  • The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.

Kit Components

  • Na Assay Buffer
  • Substrate
  • BetaG
  • DTT (1 M)
  • Na Developer
  • Na Standard (1.5 M)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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