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Anti-Osteopontin Antibody (CAB14062)

SKU:
CAB14062
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
€139 - €419
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Description

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Antibody Name:Anti-Osteopontin Antibody
Antibody SKU:CAB14062
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 106-205 of human Osteopontin (NP_001035147.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:NCI-H460, HT-1080
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 106-205 of human Osteopontin (NP_001035147.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NDSD DVDD TDDS HQSD ESHH SDES DELV TDFP TDLP ATEV FTPV VPTV DTYD GRGD SVVY GLRS KSKK FRRP DIQY PDAT DEDI TSHM ESEE LNGA YKAI
Gene ID:6696
Uniprot:P10451
Cellular Location:Secreted
Calculated MW:32kDa/33kDa/35kDa
Observed MW:33kDa/70kDa
Synonyms:BNSP, BSPI, ETA-1, OPN, Osteopontin, SPP1
Background:The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: extracellular space; cell projection; apical part of cell; perinuclear region of cytoplasm; extracellular region

Molecular Function:protein binding; extracellular matrix binding; cytokine activity

Biological Process: osteoblast differentiation; extracellular matrix disassembly; neutrophil chemotaxis; extracellular matrix organization and biogenesis; response to vitamin D; biomineral formation; response to steroid hormone stimulus; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; decidualization; cell adhesion; inflammatory response; embryo implantation

NCBI Summary:The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:P10451
NCBI GenInfo Identifier:129260
NCBI Gene ID:6696
NCBI Accession:P10451.1
UniProt Secondary Accession:P10451,Q15681, Q15682, Q15683, Q4W597, Q567T5, Q8NBK2 Q96IZ1, B2RDA1,
UniProt Related Accession:P10451
Molecular Weight:33,843 Da
NCBI Full Name:Osteopontin
NCBI Synonym Full Names:secreted phosphoprotein 1
NCBI Official Symbol:SPP1  
NCBI Official Synonym Symbols:OPN; BNSP; BSPI; ETA-1  
NCBI Protein Information:osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
UniProt Protein Name:Osteopontin
UniProt Synonym Protein Names:Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
Protein Family:Osteopontin
UniProt Gene Name:SPP1  
UniProt Entry Name:OSTP_HUMAN
Anti-Osteopontin Antibody (CAB14062)Western blot analysis of extracts of various cell lines, using Osteopontin antibody (CAB14062) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

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