Starch Colorimetric/Fluorometric Assay Kit (BN00871)

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SKU:
BN00871
€565

Description

ELISA Kit Technical ManualMSDS

Starch Colorimetric/Fluorometric Assay Kit

Starch is a complex carbohydrate consisting of a large number of glucose units. All plants contain starch, present as amylose, (linear 1,4 linked polymer) and amylopectin, (highly 1,6 branched 1,4 polymer). Starch generally contains 0-25% amylose and 75-100% amylopectin. The Starch Assay Kit provides an easy, accurate assay to measure starch levels in a variety of samples. In the assay, starch is hydrolyzed to glucose which is oxidized to generate color (570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay can detect starch at 0.0004 to 2 mg/ml.

Figure 1: Starch Standard Curve: Different types of pure starch were extracted with 10N KOH/H3PO4 as described following the kit protocol.

Key Information Description

Product SKU

BN00871

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

The assay can detect starch at 0.0004 to 2 mg/ml.

Features and Benefits

  • Simple procedure; takes ~ 40 minutes
  • Fast and convenient
  • Kit contains all necessary reagents for accurate measurement of Starch levels

Kit Components

  • Hydrolysis Buffer
  • Development Buffer
  • GenieRed Probe DMSO (anhydrous)
  • Hydrolysis Enzyme Mix
  • Development Enzyme Mix
  • Starch Standard (2.0 mg/ml)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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