Succinate Dehydrogenase Activity Colorimetric Assay Kit (BN00884)

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BN00884
  • Succinate Dehydrogenase Activity Colorimetric Assay Kit (BN00884)
  • Succinate Dehydrogenase Activity Colorimetric Assay Kit (BN00884)
  • Succinate Dehydrogenase Activity Colorimetric Assay Kit (BN00884)
€792

Description

ELISA Kit Technical ManualMSDS

Succinate Dehydrogenase Activity Colorimetric Assay Kit

Succinate Dehydrogenase (SDH) (EC 1.3.5.1) or succinate-coenzyme Q reductase (SQR) or respiratory complex II is an enzyme complex, which is bound to the inner mitochondrial membrane. SDH participates in both the citric acid cycle and electron transport chain. In mammals and many bacteria, SDH consists of 2 hydrophilic subunits, SDHA (flavoprotein) and SDHB (iron-sulfur protein) and 2 hydrophobic membrane anchor subunits: SDHC and SDHD. SDH oxidizes succinate to fumarate and transfers the electrons to ubiquinone. SDH deficiency in humans leads to a variety of phenotypes including Leigh syndrome, a neurometabolic disorder, tumor formation, and myopathy. Recent studies show that SDH can prevent the generation of ROS (reactive oxygen species); therefore, measurement of succinate dehydrogenase activity has wide applications. Assay Genie's Succinate Dehydrogenase Activity Assay kit is rapid, simple and high-throughput adaptable. In this assay, Succinate dehydrogenase converts succinate to fumarate, and transfers the electron to an artificial electron acceptor (Probe), which changes the color from blue to a colorless product (depending upon the sample enzymatic activity). This assay kit can detect less than 0.1mU Succinate Dehydrogenase Activity in a variety of samples.

Figure: (a) DCIP Standard Curve (b) Measurement of Succinate Dehydrogenase Activity in Positive Control (22 µg) (c) Mitochondria isolated from mouse heart (24 µg) and liver (70 µl).

Key Information Description

Product SKU

BN00884

Size

100 assays

Detection Methods

Absorbance (OD = 600 nm)

Applications

  • Measurement of Succinate Dehydrogenase Activity in various tissues/cells.
  • Analysis of citric acid cycle.

Features and Benefits

  • Simple & rapid
  • High-throughput adaptable
  • This assay kit can detect less than 0.1mU Succinate Dehydrogenase Activity in a variety of samples

Kit Components

  • SDH Assay Buffer
  • SDH Substrate Mix (Lyophilized)
  • SDH Probe
  • DCIP Standard (2 mM)
  • SDH Positive Control (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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