Succinic acid (C4H6O4) is distributed in all plants and animal tissues and was first obtained from amber. Due to its low toxicity, it is widely used in agriculture, food and pharmaceutical industry. Succinate (C4H4O4) is the salt or ester of succinic acid. It is one of the most active components of cellular respiratory and intracellular energy generation. Measurement of succinate or succinic acid level is a key to analysis of the citric acid cycle. Assay Genie's Succinate (Succinic Acid) Assay kit is a sensitive, fast and easy-to-use kit. In this assay, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions & reduces a colorless probe to a colored product with strong absorbance at 450 nm. This assay kit can detect less than 40 uM of succinate or succinic acid in a variety of sample types.
|Detection Method:||Absorbance (450 nm)|
|Applications:||Measurement of succinate or succinic acid in various samplesAnalysis of citric acid cycle in various tissues/cells|
|Features & Benefits:||Simple, rapid & convenientThe assay can detect less than 40 uM of Succinate or Succinic Acid in various sample types.|
|Kit Components:||Succinate Assay BufferSuccinate Converter (Lyophilized), Succinate Enzyme Mix (Lyophilized), Succinate Substrate Mix (Lyophilized), Succinate Developer (Lyophilized), Succinate Standard (Lyophilized)|
|Shipping Conditions:||Gel Pack|
|USAGE:||For Research Use Only! Not For Use in Humans.|