Succinic acid (C4H6O4) is distributed in all plants and animal tissues and was first obtained from amber. Due to its low toxicity, it is widely used in agriculture, food and pharmaceutical industry. Succinate (C4H4O4) is the salt or ester of succinic acid. It is one of the most active components of cellular respiratory and intracellular energy generation. Measurement of succinate or succinic acid level is a key to analysis of the citric acid cycle. Assay Genie's Succinate (Succinic Acid) Assay kit is a sensitive, fast and easy-to-use kit. In this assay, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions & reduces a colorless probe to a colored product with strong absorbance at 450 nm. This assay kit can detect less than 40 µM of succinate or succinic acid in a variety of sample types.

Figure 1: (a) Succinate Standard Curve. (b) Measurement of Succinate levels in rat liver (40 µg), kidney (40 µg) and muscle (40 µg), jurkat Cell lysate (200 µg) and white wine (4 µl, wine sample was diluted 1:1 with 0.5 M Tris HCl, pH 8.0). Assays were performed following the kit protocol.