Succinate (Succinic Acid) Colorimetric Assay Kit (BN00873)

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BN00873
  • Succinate (Succinic Acid) Colorimetric Assay Kit (BN00873)
  • Succinate (Succinic Acid) Colorimetric Assay Kit (BN00873)
€942

Description

ELISA Kit Technical ManualMSDS

Succinate (Succinic Acid) Colorimetric Assay Kit

Succinic acid (C4H6O4) is distributed in all plants and animal tissues and was first obtained from amber. Due to its low toxicity, it is widely used in agriculture, food and pharmaceutical industry. Succinate (C4H4O4) is the salt or ester of succinic acid. It is one of the most active components of cellular respiratory and intracellular energy generation. Measurement of succinate or succinic acid level is a key to analysis of the citric acid cycle. Assay Genie's Succinate (Succinic Acid) Assay kit is a sensitive, fast and easy-to-use kit. In this assay, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions & reduces a colorless probe to a colored product with strong absorbance at 450 nm. This assay kit can detect less than 40 µM of succinate or succinic acid in a variety of sample types.

Figure 1: (a) Succinate Standard Curve. (b) Measurement of Succinate levels in rat liver (40 µg), kidney (40 µg) and muscle (40 µg), jurkat Cell lysate (200 µg) and white wine (4 µl, wine sample was diluted 1:1 with 0.5 M Tris HCl, pH 8.0). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00873

Size

100 assays

Detection Methods

Absorbance (450 nm)

Applications

  • Measurement of succinate or succinic acid in various samples
  • Analysis of citric acid cycle in various tissues/cells

Features and Benefits

  • Simple, rapid & convenient
  • The assay can detect less than 40 µM of Succinate or Succinic Acid in various sample types.

Kit Components

  • Succinate Assay Buffer
  • Succinate Converter (Lyophilized)
  • Succinate Enzyme Mix (Lyophilized)
  • Succinate Substrate Mix (Lyophilized)
  • Succinate Developer (Lyophilized)
  • Succinate Standard (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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