ECL Western Blot Substrate/Detection Kit (BN01041)

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SKU:
BN01041
€236

Description

ELISA Kit Technical ManualMSDS

ECL Western Blot Substrate/Detection Kit

Assay Genie's SuperBrite ECL kit includes second-generation ECL substrates. They are highly sensitive, non-radioactive, and enhanced luminol-based chemiluminescent substrates for easy detection of horseradish peroxidase (HRP) on immunoblots. Blots can be repeatedly exposed to X-ray film or a CCD camera to obtain optimal results or stripped off the immune-detection reagents and re-probed.

Figure: Comparison of Assay Genie’s second-generation ECL substrate with competitor’s ECL substrate: Western Blot of protein Procathepsin K was performed using this kit (A) and competitor’s kit (B). The protein was separated on a 17% SDS-PAGE and transferred to PVDF membrane. The blot was incubated with 4ug/ml anti-rabbit Procathepsin K antibody overnight at 4°C, followed by incubation with HRP-conjugated goat anti-Rabbit IgG at 1:5,000 dilution for 1 hr at room temperature. The membrane was exposed to G:BOX for 3 min. Lane 1: 1 ng; Lane 2: 2 ng of the Procathepsin K. Dot Blot of HRP using this kit (C) and competitor’s kit (D). HRP was spotted on PVDF membrane starting at 10 ng and serially diluted 1:1 subsequently. The membrane was exposed to G:BOX for 3 min. Western Blot of
protein SAHH was performed using this kit (E) and competitor’s kit (F). The protein was separated on 8% SDS-PAGE and transferred to PVDF membrane. Sample Loading: 0.1, 0.2, 0.5, 1, 2, 5, and 10 ng (from left to right). The blot was incubated with 4 ug/ml anti-rabbit SAHH antibody overnight at 4°C, followed by incubation with HRP-conjugated goat anti-Rabbit IgG at 1:5,000 dilution for 1 hr at room temperature. The membrane was exposed to G:BOX for 3 min. (E) and 10 min. (F).

Key Information Description

Product SKU

BN01041

Size

50 ml

Detection Method

Western blot. Dot blot

Applications

Western blot. Dot blot

Features and Benefits

  • 50 ml SuperBrite ECL Western Blotting solutions, sufficient for 30 min-gel blots or 2500 cm2 of membrane
  • Minimal volumes required: Can be used for 30 mini-gel blots or 2500 cm2 of membrane.
  • Exceptional Sensitivity: Detects targets in the low picogram levels. Multiple imaging options: Compatible with X-Ray, CCD and laser based imaging.
  • Ready to Use: No additional reconstitutions required.
  • Stable signal: Signals developed are stable.
  • Economical: Available at the most competitive rates.

Kit Components

  • Reagent A
  • Reagent B

Features and Benefits

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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