GenieGlow Highly Stable Luciferase/Luciferin Reagent (BN01003)

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BN01003
  • GenieGlow Highly Stable Luciferase/Luciferin Reagent (BN01003)
  • GenieGlow Highly Stable Luciferase/Luciferin Reagent (BN01003)
  • GenieGlow Highly Stable Luciferase/Luciferin Reagent (BN01003)
  • GenieGlow Highly Stable Luciferase/Luciferin Reagent (BN01003)
€1,002 - €6,162

Description

ELISA Kit Technical ManualMSDS

GenieGlow Highly Stable Luciferase/Luciferin Reagent

Assay Genie's Highly Stable Luciferase/Luciferin Reagent utilizes a genetically modified variant derived from the Luciferase of Diaphanes pectinealis (Chinese Firefly) endemic to Yunnan province, China. Assay Genie's luciferase (rLucHS) has been modified to provide enhanced stability compared to the normal phenotype of Photinus pyralis, as well as a broader and more physiologically relevant effective pH range. At all pHs below ~8.2, rLucHS is significantly more active than commercial Photinus pyralis luciferase and is stable for weeks at room temperature and >60 minutes at 37°C. The specific activity of rLucHS is ~ 5x10RLU/mg protein. This reagent has application to measure ATP levels in a wide variety of biological samples such as cells, tissues and fermentation broth, etc.

Figure 1: 1. pH vs activity curve 2. Stadard Curve for ATP (log/log) obtained using kit protocol.

Figure 2: (3A) rLuvHS Stability at RT and 37°C (3B) vs. Standard Commercial Luciferase

Key Information Description

Detection Method

Luminescence

Size Options

  • 100 Assays
  • 1000 Assays
  • 10000 Assays

Reactive Species

Mammalian

Applications

The activity profile shows a linear detection range for ATP values as low as 10 fmol/assay (1 nM).

Features and Benefits

Stable for >10 hours at room temperature and >60 minutes at 37°C.

Kit Components

  • SuperBrite Reconstitution Buffer
  • rLucHS Reagent Mix

Storage Conditions

-80°C

Shipping Conditions

Dry Ice

USAGE

For Research Use Only! Not For Use in Humans.

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