Anti-SYNCRIP Antibody (CAB7219)

Antibody Name:	Anti-SYNCRIP Antibody
Antibody SKU:	CAB7219
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SYNCRIP (NP_001153146.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, A-431, MCF7, BT-474, Mouse heart, Mouse brain, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SYNCRIP (NP_001153146.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATE HVNG NGTE EPMD TTSA VIHS ENFQ TLLD AGLP QKVA EKLD EIYV AGLV AHSD LDER AIEA LKEF NEDG ALAV LQQF KDSD LSHV QNKS AFLC GVMK TYRQ REKQ GTKV ADSS KGPD EAKI KALL ERTG YTLD VTTG QRKY GGPP PDSV YSGQ QPSV GTEI FVGK IPRD LFED ELVP LFEK AGPI WDLR LMMD PLTG
Gene ID:	10492
Uniprot:	O60506
Cellular Location:	Cytoplasm, Endoplasmic reticulum, Microsome, Nucleus, Nucleus, nucleoplasm
Calculated MW:	46kDa/58kDa/62kDa/65kDa/69kDa
Observed MW:	50-100kDa

Synonyms:

SYNCRIP, GRY-RBP, GRYRBP, HNRNPQ, HNRPQ1, NSAP1, PP68, hnRNP-Q

Background:

This gene encodes a member of the cellular heterogeneous nuclear ribonucleoprotein (hnRNP) family. hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. The encoded protein plays a role in multiple aspects of mRNA maturation and is associated with several multiprotein complexes including the apoB RNA editing-complex and survival of motor neurons (SMN) complex. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 20.

UniProt Protein Function:	NSAP1: a heterogeneous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. A component of the spliceosome. Five alternatively spliced isoforms have been described. Isoform 1, 2 and 3 are associated with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to ACF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins.
UniProt Protein Details:	Protein type:RNA splicing; Motility/polarity/chemotaxis; Spliceosome; RNA-binding Chromosomal Location of Human Ortholog: 6q14-q15 Cellular Component: membrane; nucleus; ribonucleoprotein complex Molecular Function:protein binding; RNA binding Biological Process: negative regulation of translation; nuclear mRNA splicing, via spliceosome; osteoblast differentiation; RNA processing; RNA splicing
NCBI Summary:	This gene encodes a member of the cellular heterogeneous nuclear ribonucleoprotein (hnRNP) family. hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. The encoded protein plays a role in multiple aspects of mRNA maturation and is associated with several multiprotein complexes including the apoB RNA editing-complex and survival of motor neurons (SMN) complex. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 20. [provided by RefSeq, Dec 2011]
UniProt Code:	O60506
NCBI GenInfo Identifier:	92090361
NCBI Gene ID:	10492
NCBI Accession:	O60506.2
UniProt Secondary Accession:	O60506,Q53H05, Q5TCG2, Q5TCG3, Q8IW78, Q8N599, Q96LC1 Q96LC2, Q9Y583, E1P501, E1P502,
UniProt Related Accession:	O60506
Molecular Weight:	46,328 Da
NCBI Full Name:	Heterogeneous nuclear ribonucleoprotein Q
NCBI Synonym Full Names:	synaptotagmin binding cytoplasmic RNA interacting protein
NCBI Official Symbol:	SYNCRIP
NCBI Official Synonym Symbols:	PP68; NSAP1; GRYRBP; HNRNPQ; HNRPQ1; GRY-RBP; hnRNP-Q
NCBI Protein Information:	heterogeneous nuclear ribonucleoprotein Q
UniProt Protein Name:	Heterogeneous nuclear ribonucleoprotein Q
UniProt Synonym Protein Names:	Glycine- and tyrosine-rich RNA-binding protein; GRY-RBP; NS1-associated protein 1; Synaptotagmin-binding, cytoplasmic RNA-interacting protein
Protein Family:	Heterogeneous nuclear ribonucleoprotein
UniProt Gene Name:	SYNCRIP
UniProt Entry Name:	HNRPQ_HUMAN

	Western blot analysis of extracts of various cell lines, using SYNCRIP antibody (CAB7219) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human liver cancer using SYNCRIP antibody (CAB7219) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse Intestine using SYNCRIP antibody (CAB7219) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using SYNCRIP antibody (CAB7219) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A-549 cells using SYNCRIP antibody (CAB7219). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-hnRNP Q Antibody (CAB9609)	hnRNP Q Colorimetric Cell-Based ELISA
SYNCRIP Antibody (PACO06559)
SYNCRIP Antibody (PACO12598)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)