Anti-TAAR5 Antibody (PACO55798)

Antibody Name:	TAAR5 Antibody (PACO55798)
Antibody SKU:	PACO55798
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500
Species Reactivity:	Human, Rat, Mouse
Immunogen:	Recombinant Human Trace amine-associated receptor 5 protein (1-34AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Rat brain tissue, Mouse brain tissue. All lanes: TAAR5 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 kDa. Observed band size: 39 kDa.
	Immunofluorescence staining of Hela cells with PACO55798 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO55798 diluted at 1:600 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Olfactory receptor specific for trimethylamine, a trace amine. Also activated at lower level by dimethylethylamine. Trimethylamine is a bacterial metabolite found in some animal odors, and to humans it is a repulsive odor associated with bad breath and spoiled food. This receptor is probably mediated by the G(s)-class of G-proteins which activate adenylate cyclase.
Synonyms:	Trace amine-associated receptor 5 (TaR-5) (Trace amine receptor 5) (hTaar5) (Putative neurotransmitter receptor), TAAR5, PNR

UniProt Protein Function:	TAAR5: Orphan receptor. Ligands are likely small molecules, either sharing some similarities with trace amine as, e.g. derivatives of indolamines (such as 5-methoxytryptamine) or of phenylethylamines (such as phenylethanolamine) or being any kind of metabolite of amino acids or biogenic amine neurotransmitters. Belongs to the G-protein coupled receptor 1 family.Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1Chromosomal Location of Human Ortholog: 6q23Cellular Component: integral to membrane; integral to plasma membraneMolecular Function: G-protein coupled receptor activityBiological Process: signal transduction
UniProt Protein Details:
NCBI Summary:
UniProt Code:	O14804
NCBI GenInfo Identifier:	82582283
NCBI Gene ID:	9038
NCBI Accession:	O14804.2
UniProt Secondary Accession:	O14804,Q2M1V1, Q4VBL1, Q5VUQ3, Q6NTA8, D8KZS1
UniProt Related Accession:	O14804
Molecular Weight:	38,242 Da
NCBI Full Name:	Trace amine-associated receptor 5
NCBI Synonym Full Names:	trace amine associated receptor 5
NCBI Official Symbol:	TAAR5
NCBI Official Synonym Symbols:	PNR
NCBI Protein Information:	trace amine-associated receptor 5
UniProt Protein Name:	Trace amine-associated receptor 5
UniProt Synonym Protein Names:	Putative neurotransmitter receptor
Protein Family:	Trace amine-associated receptor
UniProt Gene Name:	TAAR5
UniProt Entry Name:	TAAR5_HUMAN

Antibodies

Secondary Antibody

Illuminating the Multifaceted Role of Acetylation: Bridging Chemistry and Biology Introduction: