Anti-TEAD3 Antibody (CAB7454)

Antibody Name:	Anti-TEAD3 Antibody
Antibody SKU:	CAB7454
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 130-330 of human TEAD3 (NP_003205.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	BT-474, MCF7, Mouse heart, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 130-330 of human TEAD3 (NP_003205.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SSAQ IVSA SVLQ NKFS PPSP LPQA VFST SSRF WSSP PLLG QQPG PSQD IKPF AQPA YPIQ PPLP PTLS SYEP LAPL PSAA ASVP VWQD RTIA SSRL RLLE YSAF MEVQ RDPD TYSK HLFV HIGQ TNPA FSDP PLEA VDVR QIYD KFPE KKGG LKEL YEKG PPNA FFLV KFWA DLNS TIQE GPGA FYGV SSQY SSAD SMTI S
Gene ID:	7005
Uniprot:	Q99594
Cellular Location:	Nucleus
Calculated MW:	48kDa
Observed MW:	49kDa

Synonyms:	TEAD3, DTEF-1, ETFR-1, TEAD-3, TEAD5, TEF-5, TEF5
Background:	This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is predominantly expressed in the placenta and is involved in the transactivation of the chorionic somatomammotropin-B gene enhancer. Translation of this protein is initiated at a non-AUG (AUA) start codon.

UniProt Protein Function:	TEAD3: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to multiple functional elements of the human chorionic somatomammotropin-B gene enhancer.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p21.2 Cellular Component: nucleoplasm Molecular Function:protein binding; transcription factor activity Biological Process: asymmetric neuroblast division; female pregnancy; regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter
NCBI Summary:	This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is predominantly expressed in the placenta and is involved in the transactivation of the chorionic somatomammotropin-B gene enhancer. Translation of this protein is initiated at a non-AUG (AUA) start codon. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99594
NCBI GenInfo Identifier:	2501157
NCBI Gene ID:	7005
NCBI Accession:	Q99594.2
UniProt Secondary Accession:	Q99594,O95910, Q5BJG7, Q8N6Y4,
UniProt Related Accession:	Q99594
Molecular Weight:	48,676 Da
NCBI Full Name:	Transcriptional enhancer factor TEF-5
NCBI Synonym Full Names:	TEA domain transcription factor 3
NCBI Official Symbol:	TEAD3
NCBI Official Synonym Symbols:	TEF5; TEAD5; TEF-5; DTEF-1; ETFR-1; TEAD-3
NCBI Protein Information:	transcriptional enhancer factor TEF-5
UniProt Protein Name:	Transcriptional enhancer factor TEF-5
UniProt Synonym Protein Names:	DTEF-1; TEA domain family member 3; TEAD-3
Protein Family:	Transcriptional enhancer factor
UniProt Gene Name:	TEAD3
UniProt Entry Name:	TEAD3_HUMAN

	Western blot analysis of extracts of various cell lines, using TEAD3 antibody (CAB7454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human placenta using TEAD3 Rabbit pAb (CAB7454) at dilution of 1:50 (40x lens).
	Immunofluorescence analysis of A549 cells using TEAD3 antibody (CAB7454).
	Confocal immunofluorescence analysis of U-2 OS cells using TEAD3 Polyclonal Antibody (CAB7454) at dilution of 1:400. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
TEAD3 Antibody (PACO05190)	TEAD3 Transcription Factor Activity Assay
TEAD3 Antibody (PACO12691)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)