Thrombin Activity Fluorometric Assay Kit (BN00635)

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SKU:
BN00635
  • Thrombin Activity Fluorometric Assay Kit (BN00635)
  • Thrombin Activity Fluorometric Assay Kit (BN00635)
€792

Description

ELISA Kit Technical ManualMSDS

Thrombin Activity Fluorometric Assay Kit

Thrombin enzyme (Factor IIa, EC 3.4.21.5), a Serine protease, is an important clotting factor in the coagulation cascade that involves the conversion of soluble fibrinogen to insoluble active fibrin strands. In this pathway, Prothrombin is proteolytically converted into an active Thrombin. Thrombin is also a potent vasoconstrictor and mitogen implicated as a major factor in vasospasm following subarachnoid hemorrhage. Ruptured cerebral aneurysm blood clots around a cerebral artery releases thrombin, which in turn induces acute and prolonged narrowing of the blood vessel, potentially resulting in cerebral ischemia and infarction (stroke). In addition, it is a pro-inflammatory enzyme that may influence the onset and progression of atherosclerosis. Assay Genie's Thrombin activity assay kit utilizes the ability of Thrombin to proteolytically cleave a synthetic substrate and release a fluorophore, AMC, which can be easily quantified by fluorescence reader. This assay kit is simple, rapid and can detect Thrombin activity as low as 1 ng in samples.

Figure: (a) Standard plot of Thrombin activity, (b) Thrombin activity was measured in plasma samples in the presence and absence of a Thrombin inhibitor, PPACK Dihydrochloride. S = Substrate, I = Inhibitor, AB = Activation Buffer containing Factor Xa. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00635

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 350/450 nm)

Applications

  • Determine activity of pure Thrombin
  • Detect the activity of Thrombin in plasma

Features and Benefits

  • Simple & convenient protocol.
  • Can detect thrombin activity as low as.
  • Kit contains all necessary reagents for measuring thrombin activity.

Kit Components

  • Thrombin Dilution Buffer
  • Thrombin Assay Buffer
  • Thrombin Enzyme Standard
  • Thrombin Substrate

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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