Thrombin Cleavage Kit (BN00639)

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SKU:
BN00639
  • Thrombin Cleavage Kit (BN00639)
  • Thrombin Cleavage Kit (BN00639)
€768

Description

ELISA Kit Technical ManualMSDS

Thrombin Cleavage Kit

Thrombin enzyme (Factor IIa, EC 3.4.21.5) is an important clotting factor that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. It is also used as a valuable biochemical tool due to its high proteolytic specificity. Thrombin cleavage site (e.g. Leu-Val-Pro-Arg-ll-Gly-Ser; ll: cleavage site) is widely incorporated within the linker region of fusion or affinity tagged recombinant proteins. After successful cleavage with Thrombin, affinity tags or fused proteins can be separated from the target protein. Assay Genie's Thrombin Cleavage Kit provides an easy approach to test and optimize cleavage conditions of a target fusion or affinity-tagged protein containing Thrombin-specific cleavage site. The kit contains an active Thrombin enzyme sufficient to cleave up to 5 mg of the target protein. A 6xHis-tagged protein containing the Thrombin cleavage site is included as a Cleavage Control Protein. Following cleavage of the target protein, Thrombin can be removed by passing the reaction mix through the Heparin Sepharose column (Catalog # 6553 and 6554).

Figure: SDS-PAGE analysis of Cleavage Control Protein: (a) Cleavage of 10 μg of 6xHis-tagged cleavage control protein with different amounts (0.01-1 U/ μl) of Thrombin at room temperature for 18 hrs. (b) Cleavage of 10 μg of 6xHis-tagged cleavage control protein at different time intervals.

Key Information Description

Product SKU

BN00639

Size

500 assays

Detection Method

SDS-PAGE

Applications

Efficiently removes tags from recombinant fusion proteins containing accessible Thrombin-specific recognition sequence.

Features and Benefits

  • Simple procedure, fast and efficient cleavage of tags
  • The kit contains an active Thrombin enzyme sufficient to cleave up to 5 mg of target protein.
  • A 6x His-tagged protein containing the Thrombin cleavage site is included as a control.
  • Following cleavage of the target protein, Thrombin can be conveniently removed using a Heparin Sepharose column.

Kit Components

  • Thrombin Dilution Buffer
  • Thrombin Cleavage Buffer
  • Thrombin Enzyme
  • Cleavage Control Protein

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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