Thrombin Inhibitor Screening Kit (Fluorometric) (BN00636)

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  • Thrombin Inhibitor Screening Kit (Fluorometric) (BN00636)
  • Thrombin Inhibitor Screening Kit (Fluorometric) (BN00636)


ELISA Kit Technical ManualMSDS

Thrombin Inhibitor Screening Kit (Fluorometric)

Thrombin enzyme (Factor IIa) is an important clotting factor that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a serine protease (EC that catalyzes many coagulation-related reactions. Thrombin inhibitors are used as anticoagulants to prevent arterial and venous thrombosis. Some of these inhibitors are currently in clinical use while others are in clinical development. Assay Genie's Thrombin Inhibitor Screening Kit utilizes the ability of Thrombin to cleave a synthetic AMC-based peptide substrate to release AMC, which can be detected by measuring its fluorescence at Ex/Em = 350/450 nm. In the presence of Thrombin specific inhibitors, the extent of cleavage reaction is reduced or completely abolished. The loss in the fluorescence intensity can be correlated to the amount of inhibitor present in the assay solution. The kit provides a simple and rapid method to screen potential inhibitors of Thrombin.

Figure: (a) Inhibition of thrombin activity by a thrombin Inhibitor (PPACK Dihydrochloride). (b) Thrombin activity was measured in
plasma samples in the presence and absence of Thrombin Inhibitor (PPACK Dihydrochloride). S = Substrate, I = Inhibitor, AB =
Activation Buffer containing Factor Xa. Assays were performed following the kit protocol.

Key Information Description

Product SKU



100 assays

Detection Method

Fluorescence (Ex/Em = 350/450 nm)


  • Screening potential inhibitors of thrombin
  • Characterize/study thrombin inhibitors

Features and Benefits

  • Rapid & simple
  • Reliable test for screening potential inhibitors of thrombin
  • Includes thrombin Inhibitor [PPACK Dihydrochloride]

Kit Components

  • Thrombin Dilution Buffer
  • Thrombin Assay Buffer
  • Thrombin Enzyme Standard
  • Thrombin Substrate

Storage Conditions


Shipping Conditions

Gel Pack


For Research Use Only! Not For Use in Humans.

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