Total Collagen Assay Kit (Colorimetric) (BN00495)

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SKU:
BN00495
  • Total Collagen Assay Kit (Colorimetric) (BN00495)
  • Total Collagen Assay Kit (Colorimetric) (BN00495)
€708

Description

ELISA Kit Technical ManualMSDS

Total Collagen Assay Kit (Colorimetric)

Collagen is the most abundant insoluble protein found in the extracellular matrix and connective tissues. It can be found in skin, tendons, bone, cartilage, muscle, vitreous humor and ligaments, among other tissues. There are more than sixteen - well characterized types of collagens, but types I, II and III collagen comprise more than 80% content in mammals. The triple-helical structure of collagen is quite unique: it consists of a repeating pattern of a basic trimer: Glycine-Proline-Hydroxyproline. In cells, collagens are secreted as procollagens and these chains are transported into the Endoplasmic Reticulum, where, numerous post-translational modifications lead to the formation of a triple helix with disulfide bonds. Excessive production of collagen is linked to pathological conditions including liver cirrhosis, lung fibrosis, and tumor growth. Assay Genie's Collagen Assay Kit is a simple and sensitive assay to detect small amounts of collagens in a variety of samples. The assay is based on the acid hydrolysis of samples to form hydrolysates and Hydroxyproline. This released Hydroxyproline gets oxidized to form a reaction intermediate, which further in the reaction, forms a chromophore (Abs 560 nm). The assay is simple, sensitive and specific for collagen and can detect as low as 0.5 µg of collagen in a variety of samples such as tissue homogenates, biological fluids and purified proteins.

Figure: (a) Collagen Standard Curve (0-10 µg). (b) Estimation of Total Collagen Concentration in rat tissues. Rat kidney, liver, lung
and muscle samples were homogenized with ddH2O and hydrolyzed with 12 M HCl for 3 hrs at 120 °C. Precipitates were removed by centrifugation (10000 x g, 3 min.). Thirty microliters of the hydrolyzed samples were assayed and kit was performed according to the protocols. Collagen Content (mg Collagen/g wet tissue): Kidney: 2.01 ± 0.22; Liver: 0.71 ± 0.32; Lung: 3.03 ± 0.3; Muscle: 3.25 ± 0.98.

Key Information Description

Product SKU

BN00495

Size

100 assays

Detection Methods

Absorbance (OD = 560 nm).

Species Reactivity

Mammalian tissues, protein/peptide hydrolysates, serum, urine

Applications

Measurement of collagen in various sample types.

Features and Benefits

  • Simple, fast and convenient assay
  • Can detect as low as 0.5 µg of collagen in a variety of samples

Kit Components

  • Oxidation Buffer
  • Chloramine T Concentrate
  • Perchloric Acid/Isopropanol Solution
  • DMAB Concentrate (in DMSO)
  • Collagen I Standard (2 mg/ml)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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