Total Protein Assay Kit - Information
Assay Genie's Total Protein Assay Kit is based on an improved pyrogallol red-molybdate protein dye-binding assay. The color intensity at 600nm is directly proportional to the total protein concentration in the sample. This single reagent can be used for the quantitative determination of total protein in urine (proteinuria) and cerebrospinal fluid (blood brain barrier).
For quantitative determination of total protein.
Total Protein Assay Kit - Key Features
- Fast and sensitive. Assay takes 10 min. Linear detection range of 5 - 200 mg/dL protein.
- Robust and Reliable. Substances in urine have minimal interference (< 2%).
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Total Protein Assay Kit - Data Sheet
|Kit Includes||Reagent 200 mL, Standard 1 mL 100 mg/dL|
|Kit Requires||For 96-well plate assays: pipetting devices, clear flat bottom 96-well plates, and plate reader. For cuvette assays: pipetting devices, cuvettes and spectrophotometer capable of reading OD600nm.|
|Method of Detection||OD600nm (Pyrogallol Red)|
|Detection Limit||5 mg/dL|
|Samples||Urine, cerebrospinal fluid etc|
|Protocol Length||10 min|
|Storage||Store kit at 2-8°C.|
|Shelf Life||6 months|
Total protein concentration can be determined by several methods. However, protein dye-binding procedures are simple, reliable, rapid, readily automated and are becoming increasingly popular in assessing renal and blood brain barrier functions.