Trypsin Activity Colorimetric Assay Kit (BN00984)

SKU:
BN00984
  • Cell Health Assays Trypsin Activity Colorimetric Assay Kit BN00984
  • Cell Health Assays Trypsin Activity Colorimetric Assay Kit BN00984
  • Cell Health Assays Trypsin Activity Colorimetric Assay Kit BN00984
€523

Description

ELISA Kit Technical ManualMSDS

Trypsin Activity Colorimetric Assay Kit

Trypsin (EC 3.4.21.4) is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. Active trypsin predominantly cleaves peptide chains at the carboxyl side of the amino acids lysine or arginine, except when either is followed by proline. It is used for numerous biotechnological processes. In the assay, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at 405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured. The kit detects 10-100 mU (p-NA unit) trypsin in various samples.

Key Information Description

Product SKU

BN00984

Size

100 assays

Detection Method

Absorbance (405 nm)

Species Reactivity

Mammalian

Applications

The kit detects 10-100 mU (p-NA unit) trypsin in various samples.

Features and Benefits

Simple procedure; takes ~ 1-2 hours based on the levels of trypsin

Kit Components

  • Trypsin Assay Buffer
  • Trypsin Substrate (in DMSO)
  • Positive Control (lyophilized)
  • p-NA Standard (2 mM)
  • Trypsin Inhibitor (TLCK, 20 mM)
  • Chymotrypsin Inhibitor (TPCK,10 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Cellular Assay
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