Tryptophan Assay Kit (Fluorometric) (BN00787)

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BN00787
  • Tryptophan Assay Kit (Fluorometric) (BN00787)
  • Tryptophan Assay Kit (Fluorometric) (BN00787)
€732

Description

ELISA Kit Technical ManualMSDS

Tryptophan Assay Kit (Fluorometric)

Tryptophan (TRP) is one of the eight essential amino acids. In general, TRP is the least abundant amino acid in humans. Chemically, TRP side chain (indole) confers its unique fluorometric properties. External sources of tryptophan include chicken, tuna, bananas, cheese, chocolate etc. Tryptophan is mainly used for protein synthesis. Additionally, TRP serves as a building block for several metabolites including kynurenine, serotonin, tryptamine, melatonin, niacin, and NAD/NAPD. TRP is the only amino acid that can be found in blood in two forms: bound (BTRP) and free (FTRP) Tryptophan. Changes in tryptophan concentrations are directly related to a number of physiological and behavioral processes including: sleep, memory, depression, motion sickness, bipolar disorders, and schizophrenia. Assay Genie's Tryptophan Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects tryptophan concentration in biological fluids, including free and bound tryptophan in serum. The principle is based on the non-enzymatic reaction to generate a fluorophore (Ex/Em = 370/440 nm) using tryptophan as a building block. The reaction is specific and other amino acids do not interfere with the assay. The assay can detect as little as 2.5 µM of TRP in a variety of biological samples.

Figure: (a) Tryptophan Standard Curve. (b) Estimation of Tryptophan in human serum and urine. To measure total Tryptophan and free Tryptophan levels in serum, samples were deproteinized using the kit protocol. TTRP: Total Tryptophan in serum, FTRP: Free Tryptophan in serum. Serum total Tryptophan (10 µl, undiluted), serum free Tryptophan (30 µl, undiluted), and urine (25 µl, 10-fold diluted) samples were assayed following the kit protocol. Estimated concentrations of Tryptophan (in µM): total Tryptophan in serum: 44.2; free Tryptophan in serum: 13.7; bound Tryptophan in serum: 30.5; Total Tryptophan in urine: 32.6.

Key Information Description

Product SKU

BN00787

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 370/440 nm)

Applications

Measurement of Tryptophan in biological fluids, including free and bound tryptophan in serum

Features and Benefits

  • Simple Protocol Specific for Tryptophan without interference from other amino acids
  • This kit can detect as little as 2.5 µM of Tryptophan in a variety of biological samples

Kit Components

  • TRP Deproteinization Reagent
  • TRP Neutralization Solution
  • TRP Condenser
  • TRP Catalyst
  • TRP Standard (25 mM)

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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