Anti-ULK3 Antibody (CAB7587)

Antibody Name:	Anti-ULK3 Antibody
Antibody SKU:	CAB7587
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 44-240 of human ULK3 (NP_001092906.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	A-549, HeLa, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 44-240 of human ULK3 (NP_001092906.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KCVA KKSL NKAS VENL LTEI EILK GIRH PHIV QLKD FQWD SDNI YLIM EFCA GGDL SRFI HTRR ILPE KVAR VFMQ QLAS ALQF LHER NISH LDLK PQNI LLSS LEKP HLKL ADFG FAQH MSPW DEKH VLRG SPLY MAPE MVCQ RQYD ARVD LWSM GVIL YEAL FGQP PFAS RSFS ELEE KIRS NRVI ELPL RPLL S
Gene ID:	25989
Uniprot:	Q6PHR2
Cellular Location:	Cytoplasm
Calculated MW:	24kDa/53kDa/54kDa
Observed MW:	60kDa

Synonyms:	ULK3
Background:

UniProt Protein Function:	ULK3: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Other group; ULK family Chromosomal Location of Human Ortholog: 15q24.1 Cellular Component: cytoplasm Molecular Function:protein binding; protein serine/threonine kinase activity Biological Process: autophagy; negative regulation of smoothened signaling pathway; positive regulation of smoothened signaling pathway; protein amino acid autophosphorylation
UniProt Code:	Q6PHR2
NCBI GenInfo Identifier:	259016166
NCBI Gene ID:	25989
NCBI Accession:	Q6PHR2.2
UniProt Secondary Accession:	Q6PHR2,Q9NPN5, Q9UFS4, B2RXK3, B4DFT0, B4DRQ7, D3DW68
UniProt Related Accession:	Q6PHR2
Molecular Weight:	54,997 Da
NCBI Full Name:	Serine/threonine-protein kinase ULK3
NCBI Synonym Full Names:	unc-51 like kinase 3
NCBI Official Symbol:	ULK3
NCBI Protein Information:	serine/threonine-protein kinase ULK3
UniProt Protein Name:	Serine/threonine-protein kinase ULK3
UniProt Synonym Protein Names:	Unc-51-like kinase 3
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	ULK3
UniProt Entry Name:	ULK3_HUMAN

Western blot analysis of extracts of various cell lines, using ULK3 antibody (CAB7587) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.

Antibodies

ULK3 Antibody (PACO01678)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)