Anti-USP28 Antibody (PACO45633)

Antibody Name:	USP28 Antibody (PACO45633)
Antibody SKU:	PACO45633
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Ubiquitin carboxyl-terminal hydrolase 28 protein (430-580AA)

Form:	Liquid
Storage Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method:	Antigen Affinity Purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western blot. All lanes: USP28 antibody at 3.08µg/ml + Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 123, 120, 67 kDa. Observed band size: 123 kDa..
	Immunohistochemistry of paraffin-embedded human ovarian cancer using PACO45633 at dilution of 1:100.

Background:

Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.

Synonyms:

Ubiquitin carboxyl-terminal hydrolase 28 (EC 3.4.19.12) (Deubiquitinating enzyme 28) (Ubiquitin thioesterase 28) (Ubiquitin-specific-processing protease 28), USP28, KIAA1515

UniProt Protein Function:	USP28: a peptidase of the C19 family. USP28 and FBXW7 regulate Myc protein stability in response to DNA damage. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.19.12; Protease; Ubiquitin-specific protease Chromosomal Location of Human Ortholog: 11q23.2 Cellular Component: nuclear body; nucleoplasm; protein complex Molecular Function:protein binding; ubiquitin-specific protease activity Biological Process: cell proliferation; DNA damage checkpoint; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; protein deubiquitination; Ras protein signal transduction; regulation of protein stability; response to DNA damage stimulus; response to ionizing radiation
NCBI Summary:	The protein encoded by this gene is a deubiquitinase involved in the DNA damage pathway and DNA damage-induced apoptosis. Overexpression of this gene is seen in several cancers. [provided by RefSeq, Oct 2016]
UniProt Code:	Q96RU2
NCBI GenInfo Identifier:	20140700
NCBI Gene ID:	57646
NCBI Accession:	Q96RU2.1
UniProt Secondary Accession:	Q96RU2,Q6NZX9, Q9P213, B0YJC0, B0YJC1,
UniProt Related Accession:	Q96RU2
Molecular Weight:	122kDa
NCBI Full Name:	Ubiquitin carboxyl-terminal hydrolase 28
NCBI Synonym Full Names:	ubiquitin specific peptidase 28
NCBI Official Symbol:	USP28
NCBI Protein Information:	ubiquitin carboxyl-terminal hydrolase 28
UniProt Protein Name:	Ubiquitin carboxyl-terminal hydrolase 28
UniProt Synonym Protein Names:	Deubiquitinating enzyme 28; Ubiquitin thioesterase 28; Ubiquitin-specific-processing protease 28
Protein Family:	Ubiquitin carboxyl-terminal hydrolase
UniProt Gene Name:	USP28

Antibodies

Anti-USP28 Antibody (CAB10400)

USP28 Antibody (PACO13127)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)