Virus DNA/RNA Extraction Kit

(No reviews yet) Write a Review
SKU:
GN0005
€711

Description

Virus DNA/RNA Extraction Kit

The Viral Nucleic Acid Extraction Kit II was designed specifically for efficient purification of viral DNA and viral RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures. The efficient glass fiber spin column system is optimized for nucleic acid purification from a wide variety of both DNA and RNA viruses such as HBV, CMV, HCV, HIV, and HTLV. 101-109 copies of viral DNA/RNA can be purified from up to 200 µl samples within 20 minutes. The purified viral DNA/RNA can be used directly in qPCR and qRT-PCR assays.

Figure 1. Virus RNA was purified from 10E1-10E4 copy number of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) using the Virus DNA/RNA Kit (3 replications of each copy number). The purified RNA was eluted with 30 μl RNase-free Water. cDNA synthesis was carried out with a 10 μl aliquot of purified RNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 μl. A Real-time PCR assay was then performed with 3 μl of synthesized cDNA as template, primers (designed to amplify the T4 region on the RNA2 segment), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The results confirmed that virus RNA can be successfully extracted and detected from as low as 10E1 copy number of RGNNV. The average cycle threshold (Ct): 10E4 = 23.88, 10E3 = 27.72, 10E2 = 31.22, 10E1 = 34.62. The low Ct values indicate a high number of target nucleic acid in the sample.

Key Features

  • High Sensitivity: virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number!
  • Purify virus DNA or virus RNA in 20 minutes!
  • Sample Volume: up to 200 µl samples of plasma, serum, body fluids, supernatant of viral cell cultures
  • Spin Columns: glass fiber membrane optimized for virus DNA and virus RNA purification
  • Individually packaged virus spin columns and collection tubes, certified RNase and DNase-free
  • Elution Volume: 50 µl
  • Storage: dry at room temperature (15-25ºC)

Applications

RT-PCR/PCR, qPCR, qRT-PCR, Real-time PCR, Real-time RT-PCR, Automated Fluorescent DNA Sequencing, Next Generation Sequencing (NGS)

Quality Control

The quality of Viral Nucleic Acid Extraction Kit II is tested on a lot-to-lot basis according to ISO-certified quality management system by isolating viral DNA/RNA from a 200 µl serum sample.

Kit Components

VB Lysis Buffer

VB Lysis Buffer

AD Buffer

W1 Buffer

Wash Buffer

RNase-free Water

VB Columns

2 ml Collection Tubes


Figure 2. Hepatitis A Virus (HAV) RNA was extracted using the Viral Nucleic Acid Extraction Kit. The purified RNA was analyzed by electrophoresis on a 1% agarose gel.
M: 1 Kb DNA Ladder
1: HAV from original serum
2: HAV from 10X diluted serum
3: HAV from 100X diluted serum

Product Original Serum 10X Diluted Serum 100X Diluted Serum

Virus DNA/RNA Kit

2.17 x 107

4.47 x 106

8.48 x 105


Figure 3. HBV (DNA), HCV (RNA), HIV (RNA), and HTLV (RNA) were purified from 200 µl of positive clinical serum samples using the Viral Nucleic Acid Extraction Kit II. Real-time qPCR and 1-step qRT-PCR reactions were then conducted using the ABI 7300 Sequence Detection System (3 replications of each copy number). Serum samples containing various amounts of DNA/RNA viruses ranging from 10E1 to 10E6 copies/ml were successfully detected and identified. The low Ct values indicate a high number of target nucleic acid in the sample.

View AllClose