Zinc Assay Kit (Fluorometric) (BN00673)

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SKU:
BN00673
  • Zinc Assay Kit (Fluorometric) (BN00673)
  • Zinc Assay Kit (Fluorometric) (BN00673)
  • Zinc Assay Kit (Fluorometric) (BN00673)
€601

Description

ELISA Kit Technical ManualMSDS

Zinc Assay Kit (Fluorometric)

Zinc (Zn, Atomic Number: 30), is a metal with characteristics similar to Magnesium due to its size and its oxidation state of +2. This element is an essential mineral of great biological significance since many enzymes require zinc as an essential cofactor. Examples of biological roles of Zinc include signal transduction, gene expression, regulation of apoptosis, synaptic plasticity and prostate gland function. Assay Genie's Zinc Fluorometric Assay kit provides an easy and quantitative method to measure the metal ion Zinc in various biological samples. The assay is based on the ability of our proprietary probe that binds with high specificity to Zinc and becomes fluorescent. The fluorescent intensity is directly proportional to the amount of zinc, which can be quantified by measurement at Ex/Em= 435/535 nm in a microplate reader. This assay kit provides a just add-and-read, non-radioactive, and high-throughput adaptable for Zinc detection. The assay is rapid (less than 10 min) and sensitive (Limit of Detection: < 0.1 µM).

Figure: (a) Zinc Standard Curve. (b) Assay Specificity: Zinc and other mono, di and trivalent metals ions were tested to evaluate possible interferences. Interferences were found to be less than 1% when data was normalized using Zinc as 100% activity. (c) Measurement of Zinc in two Human Normal Pooled Serum samples (20 µl samples at 1:40 dilution) and two Human Normal Pooled Urine Samples (20 µl samples at 1:40 dilution). Assays were performed according to the kit protocol.

Key Information Description

Product SKU

BN00673

Size

100 assays

Detection Method

Fluorescence (Ex/Em= 435/535 nm)

Species Reactivity

Mammalian

Applications

The kit can detect 20 ng/ml (~0.1 µM).

Features and Benefits

  • Simple procedure; takes ~ 15 minutes
  • Fast and convenient
  • The assay is sensitive, stable and high-throughput adaptable
  • Applications- The kit can detect 20 ng/ml (~0.1 µM)

Kit Components

  • Zinc Assay Buffer
  • Zinc Probe (in DMSO)
  • Zinc Standard (50 mM)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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