Anti-NME6 Antibody (CAB14388)

Antibody Name:	Anti-NME6 Antibody
Antibody SKU:	CAB14388
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 9-194 of human NME6 (NP_005784.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 9-194 of human NME6 (NP_005784.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASI LRSP QALQ LTLA LIKP DAVA HPLI LEAV HQQI LSNK FLIV RMRE LLWR KEDC QRFY REHE GRFF YQRL VEFM ASGP IRAY ILAH KDAI QLWR TLMG PTRV FRAR HVAP DSIR GSFG LTDT RNTT HGSD SVVS ASRE IAAF FPDF SEQR WYEE EEPQ LRCG PVCY SPEG GVHY VAGT GGLG PA
Gene ID:	10201
Uniprot:	O75414
Cellular Location:
Calculated MW:	13kDa/18kDa/21kDa
Observed MW:	21kDa

Synonyms:	NME6, IPIA-ALPHA, NDK 6, NM23-H6, NDK6
Background:	Nucleoside diphosphate (NDP) kinases (EC 2.7.4.6), such as NME6, are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates (Mehus et al., 1999 [PubMed 10453732]).[supplied by OMIM, Jul 2010]

UniProt Protein Function:	NME6: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Inhibitor of p53-induced apoptosis. Belongs to the NDK family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.7.4.6; Nucleotide Metabolism - pyrimidine; Kinase, nucleoside diphosphate; Nucleotide Metabolism - purine; Kinase, other; Other group; NDK family Chromosomal Location of Human Ortholog: 3p21 Cellular Component: mitochondrion Molecular Function:nucleoside diphosphate kinase activity Biological Process: negative regulation of cell growth; negative regulation of mitosis
NCBI Summary:	Nucleoside diphosphate (NDP) kinases (EC 2.7.4.6), such as NME6, are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates (Mehus et al., 1999 [PubMed 10453732]).[supplied by OMIM, Jul 2010]
UniProt Code:	O75414
NCBI GenInfo Identifier:	12232627
NCBI Gene ID:	10201
NCBI Accession:	O75414.3
UniProt Secondary Accession:	O75414,Q53HM5, Q96E73, Q9BQ63, B4DGW7, B4DM99,
UniProt Related Accession:	O75414
Molecular Weight:	18,087 Da
NCBI Full Name:	Nucleoside diphosphate kinase 6
NCBI Synonym Full Names:	NME/NM23 nucleoside diphosphate kinase 6
NCBI Official Symbol:	NME6
NCBI Official Synonym Symbols:	NDK 6; NM23-H6; IPIA-ALPHA
NCBI Protein Information:	nucleoside diphosphate kinase 6
UniProt Protein Name:	Nucleoside diphosphate kinase 6
UniProt Synonym Protein Names:	Inhibitor of p53-induced apoptosis-alpha; IPIA-alpha; nm23-H6
Protein Family:	Nucleoside diphosphate kinase
UniProt Gene Name:	NME6
UniProt Entry Name:	NDK6_HUMAN

	Western blot analysis of extracts of various cell lines, using NME6 Rabbit pAb (CAB14388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.
	Immunohistochemistry of paraffin-embedded rat pancreas using NME6 antibody (CAB14388) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal using NME6 antibody (CAB14388) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using NME6 antibody (CAB14388) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)