AAK1 Antibody (PACO23021)
- SKU:
- PACO23021
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | AAK1 Antibody (PACO23021) |
| Antibody SKU: | PACO23021 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:200 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Fusion protein corresponding to N terminal 236 amino acids of Human Adaptor-associated protein kinase 1 |
| Form: | Liquid |
| Storage Buffer: | Supplied at 1.1mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol. |
| Purification Method: | Affinity purification |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Gel: 10%SDS-PAGE Lysate: 30 ug Human fetal brain tissue lysate Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 30 seconds. |
 | The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 31024 (AAK1 Antibody) at dilution 1/30, on the right is treated with the fusion protein. |
| Background: | Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. |
| Synonyms: | Adaptor-associated protein kinase 1, DKFZp686K16132, KIAA1048 |
| UniProt Protein Function: | AAK1: an NAK kinase. Plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis. |
| UniProt Protein Details: | Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, Other; Other group; NAK family Chromosomal Location of Human Ortholog: 2p14 Cellular Component: clathrin-coated vesicle; cytoplasm; extrinsic to plasma membrane; leading edge; terminal button Molecular Function:Notch binding; protein binding; protein serine/threonine kinase activity Biological Process: positive regulation of Notch signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein stabilization; regulation of protein localization |
| NCBI Summary: | Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q2M2I8 |
| NCBI GenInfo Identifier: | 300669613 |
| NCBI Gene ID: | 22848 |
| NCBI Accession: | Q2M2I8.3 |
| UniProt Secondary Accession: | Q2M2I8,Q4ZFZ3, Q53RX6, Q9UPV4, |
| UniProt Related Accession: | Q2M2I8 |
| Molecular Weight: | 93,578 Da |
| NCBI Full Name: | AP2-associated protein kinase 1 |
| NCBI Synonym Full Names: | AP2 associated kinase 1 |
| NCBI Official Symbol: | AAK1Â Â |
| NCBI Protein Information: | AP2-associated protein kinase 1 |
| UniProt Protein Name: | AP2-associated protein kinase 1 |
| UniProt Synonym Protein Names: | Adaptor-associated kinase 1 |
| Protein Family: | AP2-associated protein kinase |
| UniProt Gene Name: | AAK1Â Â |
| UniProt Entry Name: | AAK1_HUMAN |
| Antibodies | ELISA Kits |
| AAK1 Antibody (PACO00349) | Human AAK1 (AP2-associated protein kinase 1) ELISA Kit (HUFI05483) |
| AAK1 Colorimetric Cell-Based ELISA |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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