5. Was the package intact upon arrival?
6. How did you store the reagents upon arrival?
7. What was your sample(s) type?
12. At what temperature has the sample been stored?
13. Has the sample been repeatedly freeze-thawed?
15. Was the substrate transparent and colorless before used?
16. How long was the substrate incubated for?
17. At what temperature did you incubate the substrate?
18. Were the reconstituted standards or detection reagents used repeatedly?