Antibody Support
Antibody protocols & support
Free, practical guides for every antibody workflow — cell culture and lysis, western blotting, immunohistochemistry and immunoprecipitation — backed by PhD-level technical support to help your experiments succeed.
Antibody protocols & guides
Filter by technique or search for a protocol, buffer recipe or troubleshooting tip.
Explore antibody products & services
The reagents behind the protocols — validated antibodies and custom services for your research.
Primary Antibodies
Thousands of validated primary antibodies for WB, IHC, IF and flow cytometry.
ExploreAntibody Sampler Packs
Pathway-matched panels of eight antibodies in trial sizes — screen a whole pathway for less.
ExploreSecondary Antibodies
HRP, fluorescent and biotin-conjugated secondaries for every host and application.
ExploreIHC Antibodies
IHC-validated antibodies with recommended protocols and buffers.
ExploreKO-Validated Antibodies
Antibodies validated in knockout models for the highest specificity.
ExploreCustom Antibody Service
Monoclonal and polyclonal antibody development tailored to your target.
ExploreFrequently asked questions
How do I choose the right antibody for my application?
Match the antibody to your application (western blot, IHC, IF or flow cytometry), your species of interest and the available validation data. Each product page lists validated applications and species, and our technical team can recommend a target-specific antibody.
Which lysis buffer should I use?
RIPA buffer suits most whole-cell and tissue protein extractions for western blotting, while milder buffers such as NP-40 better preserve protein–protein interactions for co-IP. See our RIPA buffer recipe and co-IP guide.
Why is my western blot showing high background?
Common causes include insufficient blocking, too-high antibody concentration, inadequate washing or membrane over-exposure. Our 101 Western Blot Troubleshooting guide walks through the fixes step by step.
How do I optimise antibody dilution for IHC?
Start from the datasheet's recommended range and titrate against a positive control, and optimise antigen retrieval and blocking. Our IHC staining guide covers protocols and buffer recipes.
Are Assay Genie antibodies validated?
Many of our antibodies are knockout-validated and citable, with application and species reactivity data provided on each product page.
Can I get help designing my experiment?
Yes. Our PhD-level technical team provides pre- and post-sales support — use the technical support form and we'll help with antibody selection, protocols and troubleshooting.
Need help with your experiment?
Our PhD-level technical team can help you choose antibodies, optimise protocols and troubleshoot your results.