Color Step ELISA Kits

Color Step ELISA Kits

ColorStep ELISA

ColorStep ELISA kits: colour-coded, results in 2 hours

Sandwich ELISA kits with colour-coded reagents that change the colour of the wells at each step — so you can see every well was dosed correctly, catch pipetting errors before you read the plate, and get quantitative results in about two hours.

1,600+ ColorStep kitsCV below 6%50 µL sample
1,600+
ColorStep ELISA kits
~120 min
Standard protocol
<6%
CV
50 µL
Sample per well
Why colour-coded

Built-in protocol control for every well

See every step

Each reagent gives the wells a distinct colour, so a missed or double-dosed well stands out immediately.

Fewer failed plates

Spot a pipetting error mid-assay and correct it, instead of discovering it at the plate reader.

Easier training

New lab members can follow the protocol visually, with the same precision as experienced users.

How it works

The colour at each step

Step 1 · 60 min

Detection antibody, then standard or sample

Blue
Step 2 · 30 min

HRP-streptavidin

Orange
Step 3 · 10–20 min

TMB substrate

→Colourless to blue
Step 4

Stop solution, then read at 450 nm

→Blue to yellow

Many targets also run on a 3-step, 90-minute protocol with HRP-streptavidin added in step 1. Check the kit manual for your target.

Related ranges

More fast ELISA formats

QuickStep ELISA

A single load-and-wash, 90-minute workflow, including competitive kits for steroid hormones across many species.

Browse QuickStep kits →

All fast ELISA kits

Compare ColorStep and QuickStep side by side and find the fastest kit for your target.

Browse fast ELISA kits →

FAQs

ColorStep ELISA FAQs

What is a ColorStep ELISA kit?

A sandwich ELISA with colour-coded reagents: each reagent tints the wells a distinct colour, so you can see at a glance that every well received each step. It turns a routine protocol into a visual check that catches missed or double-dispensed wells before you read the plate.

How long does a ColorStep ELISA take?

Around 120 minutes on the standard protocol, and around 90 minutes for targets on the 3-step protocol where HRP-streptavidin is added in step 1. The protocol for each target is in its kit manual.

How precise are ColorStep kits?

ColorStep kits are released with a CV below 6%, supported by the visual confirmation of each pipetting step.

How much sample do I need?

50 µL of sample per well. Kits are validated for sample types such as serum, plasma and cell-culture supernatant; the validated matrices are listed on each product page.

How is ColorStep different from QuickStep?

Both are fast ELISA formats. ColorStep prioritises visual protocol control with colour-coded reagents in a sandwich format, while QuickStep uses a single load-and-wash step and includes competitive kits for hormones. Compare both on our fast ELISA kits page.

Which species are covered?

Human, mouse and rat, plus rabbit and other species. Use the species filter in the product listing below.

Color Step ELISA Kits Product listing