YBX1
YBX1 (Y-box binding protein 1, also known as YB-1, YB1, DBPB, NSEP-1 and MDR-NF1) is a cold shock domain protein that binds both DNA and RNA. It takes part in transcription, translation, mRNA stability, splicing and DNA repair. Browse Assay Genie's current range of YBX1 research reagents below, or read on for the biology behind the target.
YBX1 is a DNA- and RNA-binding protein involved in translational repression, RNA stabilisation, mRNA splicing, DNA repair and transcription regulation. It acts mainly as an RNA-binding protein. It binds preferentially to the 5'-[CU]CUGCG-3' RNA motif and specifically recognises transcripts modified by C5-methylcytosine (m5C). By binding m5C-containing mRNAs it recruits the mRNA stability maintainer ELAVL1 and so prevents mRNA decay. It is also part of the CRD-mediated complex that promotes MYC mRNA stability, and it modulates the interaction between mRNA and eukaryotic initiation factors to regulate translation.
YBX1 has further roles in RNA sorting and cell fate. It helps define the RNA composition of extracellular exosomes by sorting small non-coding RNAs, including tRNAs, Y RNAs, Vault RNAs and miRNAs, probably through recognition of m5C-containing RNAs. In epidermal progenitors it prevents senescence by regulating translation of a senescence-associated subset of cytokine mRNAs. It binds splice sites in pre-mRNA and influences splice site selection. It also binds TSC22D1 transcripts, inhibiting their translation and so negatively regulating TGF-beta-mediated transcription of COL1A2.
On DNA, the YBX1 protein binds promoters containing a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. MDR1 regulation is enhanced by acetylated APEX1. It promotes separation of DNA strands carrying mismatches or cisplatin modifications and has endonucleolytic activity, suggesting a role in DNA repair. The YBX1 gene encodes a highly conserved protein that is a component of messenger ribonucleoprotein (mRNP) complexes and may contribute to microRNA processing. It is found in the cytoplasm, nucleus, P-bodies and cytoplasmic granules, and can be secreted through non-classical pathways, where it acts as an extracellular mitogen that stimulates cell migration and proliferation. Aberrant expression is associated with cancer proliferation in numerous tissues and may be a prognostic marker for poor outcome and drug resistance in certain cancers.
YBX1 recognises m5C-modified mRNAs and recruits ELAVL1 to prevent decay, making it a focus for studies of post-transcriptional regulation.
Aberrant YBX1 expression is linked to cancer proliferation and may mark poor outcome and drug resistance in certain cancers.
YBX1 helps define which small non-coding RNAs, including tRNAs, Y RNAs and miRNAs, are sorted into extracellular exosomes.
YBX1 binds Y-box promoters such as MDR1, separates mismatched DNA strands and has endonucleolytic activity relevant to repair.
YBX1 is a DNA- and RNA-binding protein that regulates translation, mRNA stability, pre-mRNA splicing, transcription and DNA repair. It binds m5C-modified mRNAs and recruits ELAVL1 to stabilise them. A secreted form acts as an extracellular mitogen, stimulating cell migration and proliferation.
YBX1 is also called YB-1, YB1, DBPB, NSEP-1, NSEP1, MDR-NF1, BP-8, CSDB and CSDA2. Its previous HGNC name was nuclease sensitive element binding protein 1. The gene undergoes alternate splicing, giving multiple transcript variants, and pseudogenes occur on several chromosomes.
YBX1 is a highly conserved cold shock domain protein, with orthologues named Ybx1 in mouse and rat. Aberrant expression is associated with cancer proliferation in numerous tissues, and it may be a prognostic marker for poor outcome and drug resistance in certain cancers. Check the datasheet of any product for its validated reactivity.
Other Y-box proteins, RNA-binding and mRNA stability factors, and cancer-associated targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P67809; product specifications from Assay Genie datasheets.