ALDH9A1
ALDH9A1 (aldehyde dehydrogenase 9 family member A1) is a cytosolic NAD-dependent aldehyde dehydrogenase, also known as 4-trimethylaminobutyraldehyde dehydrogenase. It oxidises amino aldehydes derived from biogenic amines and polyamines, and has also been called E3, ALDH7, ALDH4 and ALDH9. Browse Assay Genie's current range of ALDH9A1 research reagents below, or read on for the biology behind the target.
ALDH9A1 converts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine with high efficiency in vitro. It can also catalyse the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction, although with low efficiency. Its physiological emphasis is on aldehydes arising from biogenic amines and polyamines.
The enzyme has high activity towards gamma-aminobutyraldehyde and other amino aldehydes. It catalyses the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid (GABA). The active enzyme is a tetramer of identical subunits of about 54 kDa, and it is located in the cytosol. Orthologues are annotated in mouse and rat, both named Aldh9a1, which allows comparative work in rodent models.
The ALDH9A1 gene encodes a member of the aldehyde dehydrogenase family, classed by HGNC in the aldehyde dehydrogenases gene group. Its previous name was aldehyde dehydrogenase 9 family, member A1, and the symbols E3, ALDH7, ALDH4 and ALDH9 appear in older literature. Researchers studying ALDH9A1 often work with an ALDH9A1 antibody or ALDH9A1 ELISA kit to examine expression of the ALDH9A1 protein, and compare it with other ALDH isozymes that act on related aldehyde substrates.
ALDH9A1 oxidises aldehydes arising from biogenic amines and polyamines, making it a relevant enzyme for studying how these aldehydes are cleared.
The enzyme dehydrogenates gamma-aminobutyraldehyde to GABA, so it is of interest in work on alternative routes to this product.
ALDH9A1 converts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine with high efficiency in vitro, supporting studies of this oxidation step.
As a member of the aldehyde dehydrogenase family with broad but low-efficiency activity, it is useful for comparing isozyme substrate preferences.
ALDH9A1 is an NAD-dependent aldehyde dehydrogenase. It converts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine with high efficiency in vitro and oxidises gamma-aminobutyraldehyde to GABA. It can also oxidise a broad range of aldehydes, though with low efficiency, including those from biogenic amines and polyamines.
UniProt names the protein 4-trimethylaminobutyraldehyde dehydrogenase, and its EC number is 1.2.1.47. HGNC lists the aliases E3, ALDH7, ALDH4 and ALDH9. The earlier gene name was aldehyde dehydrogenase 9 family, member A1. The enzyme is a tetramer of identical subunits.
Yes. Both mouse and rat have an orthologue named Aldh9a1, so the gene can be studied in rodent models. Check the datasheet of any product for its validated reactivity before using it with a given species.
Other members of the aldehyde dehydrogenase family with products at Assay Genie, useful for comparing aldehyde metabolism across isozymes.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P49189; product specifications from Assay Genie datasheets.