LDB1
LDB1 (LIM domain binding 1, also known as NLI or CLIM2) is a nuclear protein that binds the LIM domains of many LIM domain-containing transcription factors. By determining which partners these factors engage, it helps shape their transcriptional activity. Browse Assay Genie's current range of LDB1 research reagents below, or read on for the biology behind the target.
LDB1 binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. It may regulate the transcriptional activity of these LIM-containing proteins by determining specific partner interactions. In this way the LDB1 protein acts as a cofactor that shapes which gene expression programmes its partners drive, rather than acting as a classical DNA-binding factor itself.
The functions described for LDB1 are tied to defined partners. It plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1, and it acts synergistically with LHX1/LIM1 in axis formation and in activation of gene expression. With LMO2, it contributes to the regulation of red blood cell development, maintaining erythroid precursors in an immature state.
According to NCBI Gene, LDB1 enables LIM domain binding, RNA polymerase II-specific DNA-binding transcription factor binding and enzyme binding. It is involved in negative regulation of DNA-templated transcription and positive regulation of transcription by RNA polymerase II, is located in chromatin, and is part of the beta-catenin-TCF complex. HGNC places the LDB1 gene in the Wnt enhanceosome complex group, and the protein belongs to the LDB family. The mouse ortholog is Ldb1.
LDB1 binds LIM domain transcription factors and influences both negative and positive regulation of RNA polymerase II-dependent transcription.
LDB1 works with LHX3 and ISL1 in the development of interneurons and motor neurons.
With LMO2, LDB1 helps keep erythroid precursors in an immature state during red blood cell development.
LDB1 is part of the beta-catenin-TCF complex and acts with LHX1/LIM1 in axis formation.
LDB1 binds the LIM domains of many LIM domain-containing transcription factors and may regulate their activity by determining specific partner interactions. It contributes to interneuron and motor neuron development with LHX3 and ISL1, and to erythroid development with LMO2.
LDB1 is the HGNC symbol for LIM domain binding 1. The protein is also called LIM domain-binding protein 1, and NLI and CLIM2 are listed as aliases. Literature and datasheets may use any of these names, so it helps to search under all of them.
A mouse ortholog, Ldb1, is recorded for the human gene. The protein belongs to the LDB family. Researchers working in other species should check the datasheet of any product for its validated reactivity.
LIM domain partners, Wnt pathway components and developmental transcription factors that work with LDB1.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q86U70; product specifications from Assay Genie datasheets.