METTL9
METTL9 (methyltransferase 9, His-X-His N1(pi)-histidine, also known as DREV1) is a protein-histidine N-methyltransferase that places a methyl group on the pros nitrogen of histidine residues in substrate proteins. It is the main enzyme behind 1-methylhistidine modification in cells. Browse Assay Genie's current range of METTL9 research reagents below, or read on for the biology behind the target.
METTL9 is a protein-histidine N-methyltransferase that specifically catalyses 1-methylhistidine (pros-methylhistidine) methylation of target proteins. It acts on proteins carrying a His-x-His (HxH) motif, where 'x' is preferably a small amino acid. The enzyme methylates the second histidine of the motif and uses the first histidine as a recognition signature. According to UniProt, METTL9 constitutes the main methyltransferase for the 1-methylhistidine modification in cells.
Reported substrates include S100A9, NDUFB3, SLC39A5, SLC39A7, ARMC6 and DNAJB12. The 1-methylhistidine modification may affect the binding of zinc and other metals to these target proteins, which links METTL9 activity to metal handling by modified proteins. The protein is annotated to the endoplasmic reticulum and the mitochondrion, and NCBI Gene describes it as predicted to be involved in methylation.
The METTL9 gene belongs to the METTL9 family and sits in the HGNC groups of 7BS protein methyltransferases and histidine methyltransferases. Earlier names include methyltransferase like 9 and methyltransferase 9, His-X-His N1-histidine, and the alias DREV1 is still in use. The mouse ortholog is Mettl9. Researchers studying the METTL9 protein or gene often work on histidine methylation, substrate specificity and metal binding in modified proteins.
METTL9 is the main source of 1-methylhistidine modification in cells, making it a central enzyme for studying this post-translational mark.
The enzyme methylates the second histidine of His-x-His motifs, giving a defined system for studying recognition of small-residue HxH sequences.
1-methylhistidine modification may affect zinc and other metal binding in targets such as SLC39A5 and SLC39A7, which makes METTL9 relevant to metal homeostasis research.
METTL9 is found in the endoplasmic reticulum and mitochondrion, and its substrates include mitochondrial NDUFB3 and the chaperone DNAJB12.
METTL9 is a protein-histidine N-methyltransferase that catalyses pros-methylation (1-methylhistidine) of target proteins. It methylates the second histidine of His-x-His motifs and uses the first as a recognition signature. UniProt describes it as the main methyltransferase for 1-methylhistidine modification in cells.
METTL9 is also called DREV1. UniProt lists it as protein-L-histidine N-pros-methyltransferase, and earlier HGNC names were methyltransferase like 9 and methyltransferase 9, His-X-His N1-histidine. The current HGNC name is methyltransferase 9, His-X-His N1(pi)-histidine.
A mouse ortholog, Mettl9, is recorded in the data. Known METTL9 substrates include S100A9, NDUFB3, SLC39A5, SLC39A7, ARMC6 and DNAJB12. Check the datasheet of any product for its validated reactivity.
Other methyltransferase-like proteins that sit alongside METTL9 in methylation research.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9H1A3; product specifications from Assay Genie datasheets.