SETD7
SETD7 (SET domain containing 7, histone lysine methyltransferase), also known as SET7, SET7/9, Set9, KMT7 and KIAA1717, is a nuclear lysine methyltransferase. It monomethylates histone H3 at lysine 4 and a set of non-histone proteins, including p53/TP53. Browse Assay Genie's current range of SETD7 research reagents below, or read on for the biology behind the target.
SETD7 is a histone methyltransferase that specifically monomethylates lysine 4 of histone H3. Methylation at H3 lysine 4 is a specific tag for epigenetic transcriptional activation, and SETD7 plays a central role in activating genes such as collagenase and insulin. At the insulin promoter, SETD7 is recruited by IPF1/PDX-1, which leads to activation of transcription.
SETD7 also methylates non-histone proteins, including CGAS, GLI3, p53/TP53, TAF10 and possibly TAF7. It does so by recognising and binding a [KR]-[STA]-K motif in its substrates. Monomethylation of TAF10 at lysine 189 increases the affinity of TAF10 for RNA polymerase II. Monomethylation of p53/TP53 at lysine 372 stabilises p53 and increases p53-mediated transcriptional activation. Monomethylation of CGAS at lysine 491 promotes the interaction between SGF29 and CGAS. SETD7 also acts as a positive regulator of smoothened signalling by methylating full-length GLI3, which increases the ability of GLI3 to bind DNA.
The SETD7 gene encodes a protein that belongs to the class V-like SAM-binding methyltransferase superfamily and to the SET7 subfamily of histone-lysine methyltransferases. HGNC places it in the SET domain containing, non-histone lysine methyltransferase and MORN repeat containing gene groups. Its previous name was SET domain containing lysine methyltransferase 7. NCBI Gene annotates it with histone H3 methyltransferase, p53 binding and protein-lysine N-methyltransferase activities, and with roles in the DNA damage response and heterochromatin organisation. It is located in the chromosome and nucleolus. The mouse ortholog is Setd7.
SETD7 monomethylates histone H3 lysine 4, a mark linked to transcriptional activation, and is annotated in heterochromatin organisation.
Methylation of p53/TP53 at lysine 372 by SETD7 stabilises p53 and enhances its transcriptional activity, and SETD7 acts within the DNA damage response.
SETD7 methylates TAF10 to raise its affinity for RNA polymerase II and is recruited by IPF1/PDX-1 to the insulin promoter.
SETD7 methylates GLI3 to support smoothened signalling and methylates CGAS to promote its interaction with SGF29.
SETD7 is a lysine methyltransferase that monomethylates histone H3 at lysine 4, a mark associated with transcriptional activation. It also methylates non-histone proteins such as p53/TP53, TAF10, CGAS and GLI3, altering their stability, binding or activity.
SETD7 is also called SET7, SET7/9, Set9, KMT7 and KIAA1717. Its previous HGNC name was SET domain containing lysine methyltransferase 7. The UniProt entry is named histone-lysine N-methyltransferase SETD7, and the enzyme carries EC number 2.1.1.364.
The data identify a mouse ortholog, Setd7, so the gene is present in rodents as well as humans. Cross-reactivity depends on the individual reagent, so check the datasheet of any product for its validated reactivity.
Other SET domain histone lysine methyltransferases and epigenetic regulators with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8WTS6; product specifications from Assay Genie datasheets.