PKD2
PKD2 (polycystin 2, transient receptor potential cation channel, also known as PC2 and TRPP2) is a multi-pass membrane protein that forms a nonselective cation channel. It supports calcium transport and signalling, notably in the primary cilium of renal epithelial cells. Browse Assay Genie's current range of PKD2 research reagents below, or read on for the biology behind the target.
PKD2 forms a nonselective cation channel that can act as a homotetramer or form a heteromer with PKD1. Its behaviour depends on its subcellular localisation and on its binding partners. In the primary cilium it acts as a cation channel that prefers monovalent over divalent cations, allowing K(+), Na(+) and Ca(2+) influx with low selectivity for Ca(2+). It is involved in fluid-flow mechanosensation by the primary cilium in renal epithelium. In the endoplasmic reticulum it likely works as a K(+) channel that facilitates Ca(2+) release. The heterotetrameric PKD1/PKD2 channel is more Ca(2+)-permeable than the homomeric PKD2 channel.
PKD2 also regulates other channels, including TRPV4, TRPC1, IP3R and RYR2, and so shapes intracellular Ca(2+) signalling. With TRPV4 it forms mechano- and thermosensitive channels in the cilium. In cardiomyocytes it modulates Ca(2+) release from stimulated RYR2 receptors through direct association. It takes part in left-right axis specification by sensing nodal flow, forming a complex with PKD1L1 in cilia. Together with PKD1 it regulates cilium length. It also mediates systemic blood pressure and contributes to the myogenic response in cerebral arteries through vasoconstriction. UniProt reports the protein in the cilium membrane, endoplasmic reticulum membrane, cell membrane, basolateral cell membrane, Golgi apparatus, vesicles and extracellular exosomes.
The PKD2 gene encodes a member of the polycystin family and belongs to the EF-hand domain containing group. The protein interacts with polycystin 1 (PKD1), and the two may be partners in a common signalling cascade involved in tubular morphogenesis. Mutations in the gene are associated with autosomal dominant polycystic kidney disease type 2, which is reflected in its previous name, polycystic kidney disease 2 (autosomal dominant). The mouse ortholog is Pkd2. Other aliases include PKD4 and Pc-2.
PKD2 acts as a cation channel in the primary cilium, allowing K(+), Na(+) and Ca(2+) influx and contributing to flow-dependent signalling.
Mutations in the PKD2 gene are associated with autosomal dominant polycystic kidney disease type 2, making it central to renal cyst research.
PKD2 senses fluid flow in renal epithelium and nodal flow in left-right axis specification, with PKD1L1 in cilia.
PKD2 modulates RYR2-dependent Ca(2+) release in cardiomyocytes and contributes to blood pressure control and the myogenic response in cerebral arteries.
PKD2 forms a nonselective cation channel, either as a homotetramer or as a heteromer with PKD1. It mediates calcium-permeable cation flux, senses fluid flow in the primary cilium of renal epithelium, and regulates channels such as TRPV4, TRPC1, IP3R and RYR2 to modulate intracellular Ca(2+) signalling.
PKD2 is also called polycystin-2, PC2, Pc-2, TRPP2 and PKD4. Its HGNC name is polycystin 2, transient receptor potential cation channel, and an earlier name was polycystic kidney disease 2 (autosomal dominant). It belongs to the polycystin family.
Mutations in the PKD2 gene are associated with autosomal dominant polycystic kidney disease type 2. The mouse ortholog is Pkd2. Researchers working with other species should check the datasheet of any product for its validated reactivity.
Polycystin family members and channel partners that act alongside PKD2 in calcium signalling.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q13563; product specifications from Assay Genie datasheets.