PHF1
PHF1 (PHD finger protein 1, also known as PCL1, MTF2L2 and TDRD19C) is a Polycomb group protein of the Polycomblike family. It binds histone H3 trimethylated at lysine 36 and recruits the PRC2 complex, linking chromatin state to gene regulation and the DNA damage response. Browse Assay Genie's current range of PHF1 research reagents below, or read on for the biology behind the target.
PHF1 is a Polycomb group (PcG) protein that specifically binds histone H3 trimethylated at Lys-36 (H3K36me3) and recruits the PRC2 complex. H3K36me3 is a mark associated with transcriptional activation, so PHF1 connects an active-chromatin signal to a repressive methyltransferase complex. The effect of this recruitment on H3K27me3 is still debated. Some reports indicate that PRC2 recruitment by PHF1 promotes H3K27me3 and gene silencing, by spreading PRC2 and H3K27me3 into H3K36me3 loci. Another report indicates that PHF1 recruits PRC2 to double-strand breaks (DSBs) and inhibits PRC2 activity there.
The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex and functions in transcriptional repression of homeotic genes. It is also involved in the DNA damage response and is recruited to DSBs. Reduced PHF1 levels result in X-ray sensitivity and increased homologous recombination. PHF1 also regulates p53/TP53 stability and prolongs its turnover, possibly by binding a methylated form of p53/TP53. UniProt places it in the nucleus and at the centrosome. The PHF1 gene has a mouse ortholog, Phf1, and multiple transcript variants encode different isoforms.
PHF1 belongs to the Polycomblike family and to the HGNC groups of PHD finger proteins and Tudor domain containing proteins. Its aliases include PCL1, MTF2L2 and TDRD19C. These names reflect its Polycomblike relationship and its Tudor domain classification. Researchers studying PRC2 targeting, histone mark crosstalk or DNA repair choice use PHF1 as a model reader of H3K36me3.
PHF1 recruits the PRC2 complex to H3K36me3, making it a focus for studies of H3K27 methylation and silencing of homeotic genes.
Binding of H3K36me3, an activation mark, by a Polycomb protein raises questions about how active and repressive chromatin states interact.
PHF1 is recruited to double-strand breaks, and reduced levels cause X-ray sensitivity and increased homologous recombination.
PHF1 regulates p53/TP53 stability and prolongs its turnover, possibly by binding a methylated form of p53.
PHF1 is a Polycomb group protein that binds histone H3 trimethylated at Lys-36 and recruits the PRC2 complex. It functions in transcriptional repression of homeotic genes, is recruited to DNA double-strand breaks, and regulates p53/TP53 stability.
PHF1 is also known as PCL1, MTF2L2 and TDRD19C, and its full name is PHD finger protein 1. It belongs to the Polycomblike family. Multiple transcript variants encoding different isoforms have been reported for the gene.
Mouse has an ortholog, Phf1. The sources supplied do not describe a specific disease link. They do show that reduced PHF1 levels cause X-ray sensitivity and increased homologous recombination. Check the datasheet of any product for its validated reactivity.
Other PHD finger proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O43189; product specifications from Assay Genie datasheets.