SPINT2
SPINT2 (serine peptidase inhibitor, Kunitz type 2) is a transmembrane Kunitz-type inhibitor of several serine proteases, including the HGF activator. It is also known as Kop, HAI-2 and HAI2, and is a putative tumour suppressor. Browse Assay Genie's current range of SPINT2 research reagents below, or read on for the biology behind the target.
SPINT2 is a Kunitz-type protease inhibitor that restrains the activity of several serine proteases. Its best-described target is HGFAC (the HGF activator): by inhibiting this protease, SPINT2 prevents the formation of active hepatocyte growth factor. It also inhibits plasmin and both plasma and tissue kallikrein, and it inhibits the serine protease activity of TMPRSS13. In vitro, SPINT2 inhibits the serine protease activity of ST14 (matriptase) and PRSS8 (prostasin).
The SPINT2 gene encodes a transmembrane protein with two extracellular Kunitz domains, the structural modules that mediate inhibition of serine proteases. UniProt records the protein at the cell membrane and in the cytoplasm. Multiple transcript variants encoding different isoforms have been described for this gene. The mouse ortholog is Spint2.
The protein has been referred to as HAI-2 (also written HAI2) and Kop, and the gene was previously named serine protease inhibitor, Kunitz type, 2. SPINT2 is regarded as a putative tumour suppressor, and mutations in the gene result in congenital sodium diarrhoea. Researchers studying proteolytic cascades, growth factor activation and epithelial biology therefore use SPINT2 as a reference point, alongside its close relative SPINT1.
SPINT2 inhibits HGFAC, plasmin, kallikreins, TMPRSS13, and in vitro matriptase and prostasin, making it a useful model for serine protease control.
By inhibiting HGF activator, SPINT2 limits formation of active hepatocyte growth factor, linking it to growth factor activation studies.
SPINT2 is a putative tumour suppressor, so its expression and function are examined in studies of tumour development and progression.
Mutations in SPINT2 result in congenital sodium diarrhoea, making the gene relevant to research on this inherited intestinal disorder.
SPINT2 is a transmembrane Kunitz-type inhibitor of serine proteases. It inhibits HGFAC, preventing formation of active hepatocyte growth factor, and also inhibits plasmin, plasma and tissue kallikrein and TMPRSS13. In vitro it inhibits matriptase (ST14) and prostasin (PRSS8).
SPINT2 is also called Kop, HAI-2 and HAI2, and its UniProt name is Kunitz-type protease inhibitor 2. The gene was previously named serine protease inhibitor, Kunitz type, 2. Multiple transcript variants encoding different isoforms have been found for the gene.
Mutations in SPINT2 result in congenital sodium diarrhoea, and the gene is a putative tumour suppressor. A mouse ortholog, Spint2, exists, so rodent studies are possible; check the datasheet of any product for its validated reactivity.
A related Kunitz-type serine protease inhibitor with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O43291; product specifications from Assay Genie datasheets.